{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:54:51Z","timestamp":1786978491090,"version":"build-2736575974"},"reference-count":41,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T00:00:00Z","timestamp":1723766400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001870","name":"Fundacja na rzecz Nauki Polskiej","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001870","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2024,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    The main protease from coronaviruses and the 3C protease from enteroviruses play a crucial role in processing viral polyproteins, making them attractive targets for the development of antiviral agents. In this study, we employed a combinatorial chemistry approach\u2014HyCoSuL\u2014to compare the substrate specificity profiles of the main and 3C proteases from alphacoronaviruses, betacoronaviruses, and enteroviruses. The obtained data demonstrate that coronavirus M\n                    <jats:sup>pro<\/jats:sup>\n                    s exhibit overlapping substrate specificity in all binding pockets, whereas the 3C\n                    <jats:sup>pro<\/jats:sup>\n                    from enterovirus displays slightly different preferences toward natural and unnatural amino acids at the P4\u2010P2 positions. However, chemical tools such as substrates, inhibitors, and activity\u2010based probes developed for SARS\u2010CoV\u20102 M\n                    <jats:sup>pro<\/jats:sup>\n                    can be successfully applied to investigate the activity of the M\n                    <jats:sup>pro<\/jats:sup>\n                    from other coronaviruses as well as the 3C\n                    <jats:sup>pro<\/jats:sup>\n                    from enteroviruses. Our study provides a structural framework for the development of broad\u2010spectrum antiviral compounds.\n                  <\/jats:p>","DOI":"10.1002\/pro.5139","type":"journal-article","created":{"date-parts":[[2024,8,16]],"date-time":"2024-08-16T08:00:07Z","timestamp":1723795207000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Profiling of coronaviral M\n                    <sup>pro<\/sup>\n                    and enteroviral\n                    <scp>\n                      3C\n                      <sup>pro<\/sup>\n                    <\/scp>\n                    specificity provides a framework for the development of broad\u2010spectrum antiviral compounds"],"prefix":"10.1002","volume":"33","author":[{"given":"Wioletta","family":"Rut","sequence":"first","affiliation":[{"name":"Department of Chemical Biology and Bioimaging Wroclaw University of Science and Technology  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Groborz","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Bioimaging Wroclaw University of Science and Technology  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xinyuanyuan","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rolf","family":"Hilgenfeld","sequence":"additional","affiliation":[{"name":"Institute of Molecular Medicine University of L\u00fcbeck  L\u00fcbeck Germany"},{"name":"German Center for Infection Research (DZIF), Hamburg\u2010L\u00fcbeck\u2010Borstel\u2010Riems Site University of L\u00fcbeck  L\u00fcbeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8510-1967","authenticated-orcid":false,"given":"Marcin","family":"Drag","sequence":"additional","affiliation":[{"name":"Department of Chemical Biology and Bioimaging Wroclaw University of Science and Technology  Wroclaw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2024,8,16]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf327"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085658"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-26239-2"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13030434"},{"issue":"3","key":"e_1_2_8_6_1","first-page":"162","article-title":"SARS\u2010CoV\u20102 variant biology: immune escape, transmission and fitness","volume":"21","author":"Carabelli AM,","year":"2023","journal-title":"Nature Reviews Microbiology"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02406-09"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abb4489"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M704870200"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.abg5827"},{"key":"e_1_2_8_11_1","volume-title":"European Centre for Disease Prevention and Control. 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