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In the present report, we have studied the conformation and stability of recombinant receptor extracellular domain (RED) from the human low affinity receptor and the structural basis of its interaction with NGF. Circular dichroism (CD) studies indicate that the RED is primarily random coil in nature with little regular secondary structure. Thermal stability studies have shown that this irregular conformation is a specific structure that can undergo a reversible two\u2010state thermal denaturation with a concomitant fluorescent and CD change. During heating at 100 \u00b0C for 15 min, the structure of RED is sufficiently unfolded for a reducing agent, dithiothreitol, to inactivate the receptor toward NGF binding and cross\u2010linking. The complex formation between the RED and NGF has been examined by differential CD measurements, and we have shown that a small, reproducible change in conformation occurs in RED or NGF upon interaction. These results are interpreted in terms of the initiation of NGF cell surface binding and possible modes of signal transduction.<\/jats:p>","DOI":"10.1002\/pro.5560010808","type":"journal-article","created":{"date-parts":[[2009,2,9]],"date-time":"2009-02-09T23:43:58Z","timestamp":1234223038000},"page":"1023-1031","source":"Crossref","is-referenced-by-count":45,"title":["Spectroscopic and chemical studies of the interaction between nerve growth factor (NGF) and the extracellular domain of the low affinity NGF receptor"],"prefix":"10.1002","volume":"1","author":[{"given":"David E.","family":"Timm","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Prabhakar","family":"Vissavajjhala","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alonzo H.","family":"Ross","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenneth E.","family":"Neet","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"15509","DOI":"10.1016\/S0021-9258(17)42578-6","article-title":"Association of 125I\u2010nerve growth factor with PC12 pheochromocytoma cells: Evidence for internalization via high affinity receptors only and for long\u2010term regulation by nerve growth factor of both high and low affinity receptors","volume":"259","author":"Bernd P.","year":"1984","journal-title":"J. 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