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The conformations of three of the six loops surrounding the active site are relatively well defined in the models of \u03b3\u2010NGF and EGF\u2010BP, but three other loops are likely to have flexible conformations. Although the amino acid sequence of \u03b1\u2010NGF is closely related to those of \u03b3\u2010NGF and EGF\u2010BP, it is catalytically inactive. Model\u2010building studies on \u03b1\u2010NGF suggested that mutations (in \u03b1\u2010NGF) just prior to the active site serine (195) and an unusual N\u2010terminal sequence are consistent with \u03b1\u2010NGF having a zymogen\u2010like conformation (similar to that in chymotrypsinogen).<\/jats:p><jats:p>An hypothetical model of the quaternary structure of HMW\u2010EGF has been constructed using this model of EGF\u2010BP and the NMR structure of murine EGF. The C\u2010terminal arm of EGF was modeled into the active site of EGF\u2010BP based on data indicating that the C\u2010terminal arginine of EGF occupies the S<jats:sub>1<\/jats:sub> subsite of EGF\u2010BP. Data suggesting one of the surface loops of EGF\u2010BP is buried in the HMW\u2010EGF complex and symmetry constraints were important in deriving a schematic model. A molecular docking program was used to fit EGF to EGF\u2010BP.<\/jats:p>","DOI":"10.1002\/pro.5560020805","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T01:02:29Z","timestamp":1234227749000},"page":"1229-1241","source":"Crossref","is-referenced-by-count":12,"title":["Prediction of the three\u2010dimensional structures of the nerve growth factor and epidermal growth factor binding proteins (kallikreins) and an hypothetical structure of the high molecular weight complex of epidermal growth factor with its binding protein"],"prefix":"10.1002","volume":"2","author":[{"given":"Ben","family":"Bax","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldine","family":"Ferguson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael","family":"Blaber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael J. 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