{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T21:35:06Z","timestamp":1790199306021,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":5297,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1994,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A new family of protein domains consisting of 50\u201380 amino acid residues is described. It is composed of nearly 40 members, including domains encoded by plastid and phage group I introns; mitochondrial, plastid, and bacterial group II introns; eubacterial genomes and plasmids; and phages. The name \u201cEX<jats:sub>1<\/jats:sub>H\u2010HX<jats:sub>3<\/jats:sub>H\u201d was coined for both domain and family. It is based on 2 most prominent amino acid sequence motifs, each encompassing a pair of highly conserved histidine residues in a specific arrangement: EX<jats:sub>1<\/jats:sub>HH and HX<jats:sub>3<\/jats:sub>H. The \u201cHis\u201d motifs often alternate with amino\u2010 and carboxy\u2010terminal motifs of a new type of Zn\u2010finger\u2010like structure CX<jats:sub>2, 4<\/jats:sub>CX<jats:sub>29\u201354<\/jats:sub>[CH]X<jats:sub>2, 3<\/jats:sub>[CH]. The EX<jats:sub>1<\/jats:sub>HH\u2010HX<jats:sub>3<\/jats:sub>H domain in eubacterial E2\u2010type bacteriocins and in phage RB3 (wild variant of phage T4) product of the nrdB group I intron was reported to be essential for DNA endonuclease activity of these proteins. In other proteins, the EX<jats:sub>1<\/jats:sub>HH\u2010EX<jats:sub>3<\/jats:sub>H domain is hypothesized to possess DNase activity as well. Presumably, this activity promotes movement (rearrangement) of group I and group II introns encoding the EX<jats:sub>1<\/jats:sub>HH\u2010HX<jats:sub>3<\/jats:sub>H domain and other gene targets. In the case of <jats:italic>Escherichia coli<\/jats:italic> restrictase McrA and possibly several related proteins, it appears to mediate the restriction of alien DNA molecules.<\/jats:p>","DOI":"10.1002\/pro.5560030716","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T01:42:02Z","timestamp":1234230122000},"page":"1117-1120","source":"Crossref","is-referenced-by-count":87,"title":["Self\u2010splicing group I and group II introns encode homologous (putative) DNA endonucleases of a new family"],"prefix":"10.1002","volume":"3","author":[{"given":"Alexander E.","family":"Gorbalenya","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2836(05)80360-2"},{"key":"e_1_2_1_3_1","first-page":"127","volume-title":"Mathematical methods of analysis of biopolymer sequences.","author":"Brodsky LI","year":"1993"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/12.1Part1.387"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1086\/416128"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.5.6.1032"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/364358a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF02102483"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(83)90457-9"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.62.070193.003103"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0303-2647(93)90062-H"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(86)90651-3"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/21.22.4991"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.16.7558"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.175.10.2907-2916.1993"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.73.11.3989"},{"key":"e_1_2_1_17_1","volume-title":"MPsrch version 1.3.","author":"Sturrock SS","year":"1993"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560030716","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560030716","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,23]],"date-time":"2023-10-23T23:18:33Z","timestamp":1698103113000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560030716"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,7]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1994,7]]}},"alternative-id":["10.1002\/pro.5560030716"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560030716","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,7]]}}}