{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T12:00:42Z","timestamp":1791201642542,"version":"4.2.0"},"reference-count":24,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":5205,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1994,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The structure of the <jats:italic>Drosophila<\/jats:italic> engrailed homeodomain has been solved by molecular replacement and refined to an <jats:italic>R<\/jats:italic>\u2010factor of 19.7% at a resolution of 2.1 \u00c5. This structure offers a high\u2010resolution view of an important family of DNA\u2010binding proteins and allows comparison to the structure of the same protein bound to DNA. The most significant difference between the current structure and that of the 2.8\u2010\u00c5 engrailed\u2010DNA complex is the close packing of an extended strand against the rest of the protein in the unbound protein. Structural features of the protein not previously noted include a \u201cherringbone\u201d packing of 4 aromatic residues in the core of the protein and an extensive network of salt bridges that covers much of the helix 1\u2010helix 2 surface. Other features that may play a role in stabilizing the native state include the interaction of buried carbonyl oxygen atoms with the edge of Phe 49 and a bias toward statistically preferred side\u2010chain dihedral angles. There is substantial disorder at both ends of the 61 amino acid protein. A 51\u2010amino acid variant of engrailed (residues 6\u201356) was synthesized and shown by CD and thermal denaturation studies to be structurally and thermodynamically similar to the full\u2010length domain.<\/jats:p>","DOI":"10.1002\/pro.5560031018","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T01:20:02Z","timestamp":1234228802000},"page":"1779-1787","source":"Crossref","is-referenced-by-count":136,"title":["Structural studies of the engrailed homeodomain"],"prefix":"10.1002","volume":"3","author":[{"given":"Neil D.","family":"Clarke","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles R.","family":"Kissinger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Desjarlais","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary L.","family":"Gilliland","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carl O.","family":"Pabo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1993.1661"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767389009633"},{"key":"e_1_2_1_4_1","volume-title":"X\u2010PLOR version 3.1: A system for X\u2010ray crystallography and NMR.","author":"Br\u00fcnger A.","year":"1992"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.235.4787.458"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3892686"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767391001071"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00081a001"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889887086436"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(90)90453-L"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(94)90231-3"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"26813","DOI":"10.1016\/S0021-9258(19)74183-0","article-title":"Understanding the homeodomain","volume":"268","author":"Romberg T.","year":"1993","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889891004399"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00112a001"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(71)90324-X"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(90)91385-6"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90314-7"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90040-8"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1296"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0304-419X(89)90033-4"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8303294"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.16.4843"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767387099124"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1993.tb05776.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90526-5"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560031018","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560031018","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,27]],"date-time":"2023-09-27T21:20:36Z","timestamp":1695849636000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560031018"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1994,10]]},"references-count":24,"journal-issue":{"issue":"10","published-print":{"date-parts":[[1994,10]]}},"alternative-id":["10.1002\/pro.5560031018"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560031018","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1994,10]]}}}