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Prior to the availability of cloning techniques, the mouse submandibular gland was the richest source of NGF and provided sufficient material to enable its biochemical characterization. It binds as a dimer to at least 2 cell\u2010surface receptor types expressed in a variety of neuronal and non\u2010neuronal cells. Residues involved in these interactions and in the maintenance of tertiary and quaternary structure have been identified by chemical modification and site\u2010directed mutagenesis, and this information can be related to their location in the 3\u2010dimensional structure. For example, interactions between aromatic residues contribute to the stability of the NGF dimer, and specific surface lysine residues participate in receptor contacts. The conclusion from these studies is that receptor interactions involve broad surface regions, which may be composed of residues from both protomers in the dimer.<\/jats:p>","DOI":"10.1002\/pro.5560031102","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T01:26:04Z","timestamp":1234229164000},"page":"1901-1913","source":"Crossref","is-referenced-by-count":79,"title":["Nerve growth factor: Structure\/function relationships"],"prefix":"10.1002","volume":"3","author":[{"given":"Ralph A.","family":"Bradshaw","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Murray\u2010Rust","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom L.","family":"Blundell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil Q.","family":"Mcdonald","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Risto","family":"Lapatto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos F.","family":"Ib\u00e1\u00f1ez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-08-057132-4.50009-5"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.68.10.2417"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00779a018"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00725a018"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/0955-2235(90)90021-B"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020805"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(91)90287-A"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020804"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/3.3.153"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.64.2.787"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"8532","DOI":"10.1016\/S0021-9258(19)75252-1","article-title":"Dissociation equilibrium constant of beta nerve growth factor","volume":"252","author":"Bothwell MA","year":"1977","journal-title":"J Biol Chem"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.bi.47.070178.001203"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0968-0004(93)90052-O"},{"key":"e_1_2_1_15_1","first-page":"206","volume-title":"Endocrinology, vol 2","author":"Bradshaw RA","year":"1977"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0896-6273(92)90023-7"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(83)90078-5"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"2949","DOI":"10.1016\/S0021-9258(19)85833-7","article-title":"Oxidation of tryptophan\u201021 alters the biological activity and receptor binding characteristics of mouse nerve growth factor","volume":"255","author":"Cohen P","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1129","DOI":"10.1016\/S0021-9258(18)98144-5","article-title":"Purification and metabolic effects of nerve growth factor from snake venom","volume":"234","author":"Cohen S.","year":"1959","journal-title":"J Biol Chem"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.46.3.302"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1631557"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.63.3.885"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1098\/rspb.1991.0159"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01025171"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/jnr.490320402"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.14.5454"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"8027","DOI":"10.1016\/S0021-9258(18)47521-7","article-title":"Mouse glandular kallikrein genes. 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