{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T18:14:42Z","timestamp":1785608082724,"version":"3.56.0"},"reference-count":26,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":4962,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1995,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have developed a phage display system that provides a means to select variants of the IgG binding domain of peptostreptococcal protein L that fold from large combinatorial libraries. The premise underlying the selection scheme is that binding of protein L to IgG requires that the protein be properly folded. Using a combination of molecular biological and biophysical methods, we show that this assumption is valid. First, the phage selection procedure strongly selects against a point mutation in protein L that disrupts folding but is not in the IgG binding interface. Second, variants recovered from a library in which the first third of protein L was randomized are properly folded. The degree of sequence variation in the selected population is striking: the variants have as many as nine substitutions in the 14 residues that were mutagenized. The approach provides a selection for \u201cfoldedness\u201d that is potentially applicable to any small binding protein.<\/jats:p>","DOI":"10.1002\/pro.5560040609","type":"journal-article","created":{"date-parts":[[2010,6,29]],"date-time":"2010-06-29T08:47:45Z","timestamp":1277801265000},"page":"1108-1117","source":"Crossref","is-referenced-by-count":57,"title":["A phage display system for studying the sequence determinants of protein folding"],"prefix":"10.1002","volume":"4","author":[{"given":"Hongdi","family":"Gu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qian","family":"Yi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan T.","family":"Bray","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Riddle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Baker","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew K.","family":"Shiau","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00158a006"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8259514"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340080405"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(89)90602-7"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.4246"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1871600"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.89.9.3751"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8259512"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)42349-6"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1658931"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00132a025"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00109a004"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0378-1119(91)90583-W"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031114"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"2234","DOI":"10.1016\/S0021-9258(18)45867-X","article-title":"Protein L binds to the k light chain variable domain","volume":"267","author":"Nilson BHK","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340210103"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(91)08029-H"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.3388019"},{"key":"e_1_2_1_20_1","volume-title":"Molecular cloning: A laboratory manual, 2nd edition","author":"Sambrook J","year":"1989"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1696028"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/16.15.7583"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmra.1993.1098"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0076-6879(93)17065-D"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00251a008"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00064a023"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1051\/epn\/19861701011"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560040609","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560040609","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T21:05:09Z","timestamp":1698354309000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560040609"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1995,6]]},"references-count":26,"journal-issue":{"issue":"6","published-print":{"date-parts":[[1995,6]]}},"alternative-id":["10.1002\/pro.5560040609"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560040609","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1995,6]]}}}