{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T05:11:14Z","timestamp":1791263474121,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":4413,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1996,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The three\u2010dimensional solution\u2010 and solid\u2010state structures of the human immunodeficiency virus type\u20101 (HIV\u20101) matrix protein have been determined recently in our laboratories by NMR and X\u2010ray crystallographic methods (Massiah et al. 1994. <jats:italic>J Mol Biol 244<\/jats:italic>:198\u2013223; Hill et al. 1996. <jats:italic>Proc Natl Acad Sci USA 93<\/jats:italic>:3099\u20133104). The matrix protein exists as a monomer in solution at low millimolar protein concentrations, but forms trimers in three different crystal lattices. Although the NMR and X\u2010ray structures are similar, detailed comparisons have revealed an approximately 6 \u00c5 displacement of a short 3<jats:sub>10<\/jats:sub> helix (Pro 66\u223cGly 71) located at the trimer interface. High quality electron density and nuclear Overhauser effect (NOE) data support the integrity of the X\u2010ray and NMR models, respectively. Because matrix apparently associates with the viral membrane as a trimer, displacement of the 3<jats:sub>10<\/jats:sub> helix may reflect a physiologically relevant conformational change that occurs during virion assembly and disassembly. These findings further suggest that Pro 66 and Gly 71, which bracket the 3<jats:sub>10<\/jats:sub> helix, serve as \u201chinges\u201d that allow the 3<jats:sub>10<\/jats:sub> helix to undergo this structural reorientation.<\/jats:p>","DOI":"10.1002\/pro.5560051202","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T03:47:52Z","timestamp":1234237672000},"page":"2391-2398","source":"Crossref","is-referenced-by-count":59,"title":["Comparison of the NMR and X\u2010ray structures of the HIV\u20101 matrix protein: Evidence for conformational changes during viral assembly"],"prefix":"10.1002","volume":"5","author":[{"given":"Michael A.","family":"Massiah","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Worthylake","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Allyson M.","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wesley I.","family":"Sundquist","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christopher P.","family":"Hill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael F.","family":"Summers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0263-7855(98)80030-1"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"6487","DOI":"10.1128\/jvi.67.11.6487-6498.1993","article-title":"Functional chimeras of the Rous sarcoma virus and human immunodeficiency virus gag proteins","volume":"67","author":"Bennett RP","year":"1993","journal-title":"J Virol"},{"key":"e_1_2_1_4_1","volume-title":"X\u2010PLOR, a system for crystallography and NMR. Version 3.1","author":"Br\u00fcnger AT","year":"1992"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.2.523"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.1.367"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/365666a0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1128\/jvi.68.1.111-122.1994","article-title":"Phenotypic characterization of insertion mutants of the human immunodeficiency virus type 1 Gag precursor expressed in recombinant baculovirus\u2010infected cells","volume":"68","author":"Chazal N","year":"1994","journal-title":"J Virol"},{"key":"e_1_2_1_9_1","first-page":"34","article-title":"An automated procedure for phase improvement by density modification","volume":"51","author":"Cowtan KD","year":"1994","journal-title":"Joint CCP4 and ESF\u2010EACBM Newsletter"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.68.3.1689-1696.1994"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi952337x"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"4972","DOI":"10.1128\/jvi.67.8.4972-4980.1993","article-title":"A large deletion in the matrix domain of the human immunodeficiency virus Gag gene redirects virus particle assembly from the plasma membrane to the endoplasmic reticulum","volume":"67","author":"F\u00e4cke M","year":"1993","journal-title":"J Virol"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.69.6.3949-3954.1995"},{"key":"e_1_2_1_14_1","doi-asserted-by":"crossref","first-page":"341","DOI":"10.1128\/jvi.70.1.341-351.1996","article-title":"Domains of the human immunodeficiency virus type 1 matrix and gp41 cytoplasmic tail required for envelope incorporation into virions","volume":"70","author":"Freed EO","year":"1996","journal-title":"J Virol"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/369107b0"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1984","DOI":"10.1128\/jvi.69.3.1984-1989.1995","article-title":"Virion incorporation of envelope glycoproteins with long but not short cytoplasmic tails is blocked by specific, single amino acid substitutions in the human immunodeficiency virus type 1 matrix","volume":"69","author":"Freed EO","year":"1995","journal-title":"J Virol"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.12.4650"},{"key":"e_1_2_1_18_1","doi-asserted-by":"crossref","first-page":"5311","DOI":"10.1128\/jvi.68.8.5311-5320.1994","article-title":"Single amino acid changes in the human immunodeficiency virus type 1 matrix protein block virus particle production","volume":"68","author":"Freed EO","year":"1994","journal-title":"J Virol"},{"key":"e_1_2_1_19_1","doi-asserted-by":"crossref","first-page":"1027","DOI":"10.1128\/jvi.70.2.1027-1032.1996","article-title":"Role of karyopherin pathway in human immunodeficiency virus type I nuclear import","volume":"70","author":"Gallay P","year":"1996","journal-title":"J Virol"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90488-3"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90097-7"},{"key":"e_1_2_1_22_1","doi-asserted-by":"crossref","unstructured":"GambleTR VajdosF YooS WorthylakeDK HouseweartSM SundquistWI HillCP.1996. Crystal structure of human cyclophilin A bound to the aminoterminal domain of the HIV\u20101 capsid.Cell in press.","DOI":"10.1016\/S0092-8674(00)81823-1"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002030-199106000-00001"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90873-8"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.273.5272.231"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.86.15.5781"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.7.3099"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1089\/aid.1992.8.1"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767390010224"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889891004399"},{"key":"e_1_2_1_31_1","doi-asserted-by":"crossref","first-page":"3824","DOI":"10.1128\/jvi.69.6.3824-3830.1995","article-title":"Rescue of human immunodeficiency virus type I matrix protein mutants by envelope glycoproteins with short cytoplasmic domains","volume":"69","author":"Mammano F","year":"1995","journal-title":"J Virol"},{"key":"e_1_2_1_32_1","first-page":"112","article-title":"Computer emulation of thin section electron microscopy predicts an envelope\u2010associated icosadeltahedral capsid for human immunodeficiency virus","volume":"58","author":"Marx PA","year":"1988","journal-title":"Lab Invest"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1994.1719"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/370666a0"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/bst0230725"},{"key":"e_1_2_1_36_1","volume-title":"Human Retroviruses and AIDS","author":"Meyers G","year":"1995"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nsb0996-763"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/378743a0"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06938.x"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1990.tb17832.x"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.68.5.3232-3242.1994"},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.15.6992"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.7.4264-4273.1993"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.67.12.7067-7076.1993"},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"3804","DOI":"10.1128\/jvi.65.7.3804-3812.1991","article-title":"Suppression of retroviral MA deletions by the amino\u2010terminal membrane\u2010binding domain of p60 src","volume":"65","author":"Wills JW","year":"1991","journal-title":"J Virol"},{"key":"e_1_2_1_46_1","doi-asserted-by":"crossref","first-page":"6387","DOI":"10.1128\/jvi.67.11.6387-6394.1993","article-title":"Mutations in the N\u2010terminal region of human immunodeficiency virus type I matrix protein block intracellular transport of the Gag precursor","volume":"67","author":"Yuan X","year":"1993","journal-title":"J Virol"},{"key":"e_1_2_1_47_1","doi-asserted-by":"crossref","first-page":"4966","DOI":"10.1128\/jvi.66.8.4966-4971.1992","article-title":"The matrix protein of human immunodeficiency virus type I is required for incorporation of viral envelope protein into mature virions","volume":"66","author":"Yu X","year":"1992","journal-title":"J Virol"},{"key":"e_1_2_1_48_1","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1128\/jvi.67.1.213-221.1993","article-title":"Mutations in the cytoplasmic domain of human immunodeficiency virus type 1 transmembrane protein impair the incorporation of env proteins into mature virions","volume":"67","author":"Yu X","year":"1993","journal-title":"J Virol"},{"key":"e_1_2_1_49_1","doi-asserted-by":"crossref","first-page":"2556","DOI":"10.1128\/jvi.68.4.2556-2569.1994","article-title":"Identification of a membrane\u2010binding domain within the amino\u2010terminal region of human immunodeficiency virus type 1 Gag protein which interacts with acidic phospholipids","volume":"68","author":"Zhou W","year":"1994","journal-title":"J Virol"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560051202","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560051202","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,26]],"date-time":"2023-10-26T22:44:35Z","timestamp":1698360275000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560051202"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1996,12]]},"references-count":48,"journal-issue":{"issue":"12","published-print":{"date-parts":[[1996,12]]}},"alternative-id":["10.1002\/pro.5560051202"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560051202","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1996,12]]}}}