{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T07:35:15Z","timestamp":1762673715058},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":4201,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1997,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A crystal structure of the serine protease, mouse glandular kallikrein 13 (mGK\u201013) has been determined at 2.6\u2010\u00c5 resolution. This enzyme, isolated from the mouse submandibular gland, is also known as prorenin\u2010converting enzyme and cleaves submandibular gland Ren\u20102 prorenin to yield active renin. The mGK\u201013 structure is similar to other members of the mammalian serine protease family, having five conserved disulfide bonds and an active site located in the cleft between two \u03b2\u2010barrel domains. The mGK\u201013 structure reveals for the first time an ordered kallikrein loop conformation containing a short 3<jats:sub>10<\/jats:sub> helix. This loop is disordered in the related porcine pancreatic kallikrein and rat submandibular tonin structures. The kallikrein loop is in close spatial proximity to the active site and is also involved in a dimeric arrangement of mGK\u201013. The catalytic specificity of mCK\u201013 for Ren\u20102 prorenin was studied by modeling a prorenin\u2010derived peptide into the active site of mGK\u201013. This model emphasizes two electronegative substrate specificity pockets on the mGK\u201013 surface, which could accommodate the dibasic P2 and P1 residues at the site of prorenin cleavage by mGK\u201013.<\/jats:p>","DOI":"10.1002\/pro.5560060706","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T05:59:40Z","timestamp":1234245580000},"page":"1418-1425","source":"Crossref","is-referenced-by-count":24,"title":["The crystal structure of the mouse glandular kallikrein\u201013 (prorenin converting enzyme)"],"prefix":"10.1002","volume":"6","author":[{"given":"David E.","family":"Timm","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2008,12,31]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1982.tb07059.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(89)90110-1"},{"key":"e_1_2_1_4_1","first-page":"151","article-title":"Crystallographic analysis of mouse prorenin converting enzyme","volume":"18","author":"Blaber M.","year":"1994","journal-title":"J Cell Biochem Suppl"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00395a025"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020803"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560020804"},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","first-page":"4264","DOI":"10.1016\/S0021-9258(18)89259-6","article-title":"The refinement and the structure of the dimer of \u03b1\u2013chymotrypsin at 1.67 \u00c5 resolution","volume":"260","author":"Blevins RA","year":"1985","journal-title":"J Biol Chem"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(83)90077-3"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1989.tb08511.x"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0090-4295(99)80087-9"},{"key":"e_1_2_1_12_1","volume-title":"XPLOR version 3.1 manual","author":"Br\u00fcnger AT","year":"1992"},{"key":"e_1_2_1_13_1","volume-title":"Collaborative Computing Project, No 4: A suite of programs for protein crystallography","author":"CCP4.","year":"1979"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(83)90078-5"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(81)90186-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00395a026"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.12.6294"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(87)90658-9"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560050204"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00382a003"},{"key":"e_1_2_1_21_1","doi-asserted-by":"crossref","first-page":"16027","DOI":"10.1016\/S0021-9258(17)46181-3","article-title":"The structure of protein\u2010protein recognition sites","volume":"265","author":"Janin J","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108767390010224"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"5930","DOI":"10.1016\/S0021-9258(19)39267-1","article-title":"A processing enzyme for prorenin in mouse submandibular gland","volume":"265","author":"Kim WS","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_24_1","doi-asserted-by":"crossref","first-page":"19283","DOI":"10.1016\/S0021-9258(18)54995-4","article-title":"Mouse submandibular gland prorenin\u2010converting enzyme is a member of glandular kallikrein family","volume":"266","author":"Kim WS","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0021889891004399"},{"key":"e_1_2_1_26_1","volume-title":"Crystallographic computing","author":"Leslie AGW.","year":"1990"},{"key":"e_1_2_1_27_1","doi-asserted-by":"crossref","first-page":"7780","DOI":"10.1016\/S0021-9258(17)42861-4","article-title":"Sequence of an epidermal growth factor\u2010binding protein","volume":"259","author":"Lundgren S","year":"1984","journal-title":"J Biol Chem"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1107\/S010876818300275X"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1107\/S0108768187007559"},{"key":"e_1_2_1_30_1","doi-asserted-by":"crossref","first-page":"21027","DOI":"10.1016\/S0021-9258(17)45322-1","article-title":"Substrate specificity of prorenin converting enzyme of mouse submandibular gland","volume":"26","author":"Nakayama K","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_1_31_1","volume-title":"AMORE: A new package for molecular replacement 1\u201387\u201390","author":"Navaza J.","year":"1992"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1002\/prot.340110407"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1983.tb01624.x"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/protein\/1.5.377"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.67.1.164"},{"key":"e_1_2_1_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)42658-6"},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(85)90314-6"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.57.6.1782"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.2597"},{"key":"e_1_2_1_40_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560031116"},{"key":"e_1_2_1_41_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5560050505"}],"container-title":["Protein Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpro.5560060706","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pro.5560060706","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,28]],"date-time":"2023-10-28T01:31:42Z","timestamp":1698456702000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pro.5560060706"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1997,7]]},"references-count":40,"journal-issue":{"issue":"7","published-print":{"date-parts":[[1997,7]]}},"alternative-id":["10.1002\/pro.5560060706"],"URL":"https:\/\/doi.org\/10.1002\/pro.5560060706","archive":["Portico"],"relation":{},"ISSN":["0961-8368","1469-896X"],"issn-type":[{"value":"0961-8368","type":"print"},{"value":"1469-896X","type":"electronic"}],"subject":[],"published":{"date-parts":[[1997,7]]}}}