{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T22:16:37Z","timestamp":1791152197934,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2008,12,31]],"date-time":"2008-12-31T00:00:00Z","timestamp":1230681600000},"content-version":"vor","delay-in-days":3958,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["GM45614"],"award-info":[{"award-number":["GM45614"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[1998,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Carbonic anhydrase IV (CAIV) is a membrane\u2010associated enzyme anchored to plasma membrane surfaces by a phosphatidylinositol glycan linkage. We have determined the 2.8\u2010\u00c5 resolution crystal structure of a truncated, soluble form of recombinant murine CAIV. We have also determined the structure of its complex with a drug used for glaucoma therapy, the sulfonamide inhibitor brinzolamide (Azopt\u2122). The overall structure of murine CAIV is generally similar to that of human CAIV; however, some local structural differences are found in the active site resulting from amino acid sequence differences in the \u201c130\u2032s segment\u201d and the residue\u201063 loop (these may affect the nearby catalytic proton shuttle, His\u201064). Similar to human CAIV, the C\u2010terminus of murine CAIV is surrounded by a substantial electropositive surface potential that may stabilize the interaction with the phospholipid membrane. Binding interactions observed for brinzolamide rationalize the generally weaker affinity of inhibitors used in glaucoma therapy toward CAIV compared with CAII.<\/jats:p>","DOI":"10.1002\/pro.5560070303","type":"journal-article","created":{"date-parts":[[2010,7,12]],"date-time":"2010-07-12T06:28:21Z","timestamp":1278916101000},"page":"556-563","source":"Crossref","is-referenced-by-count":87,"title":["Structures of murine carbonic anhydrase IV and human carbonic anhydrase II complexed with brinzolamide: Molecular basis of isozyme\u2010drug discrimination"],"prefix":"10.1002","volume":"7","author":[{"given":"Travis","family":"Stams","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yun","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David W.","family":"Christianson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. 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