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Human XPA, a 31 kDa, zinc\u2010associated protein, is thought to play a major NER role in the recognition of damaged DNA and the recruitment of other proteins, including RPA, ERCC1, and TFIIH, to repair the damage. Sequence analyses and genetic evidence suggest that zinc is associated with a C4\u2010type motif, C10S\u2010X<jats:sub>2<\/jats:sub>\u2010C108\u2010X<jats:sub>17<\/jats:sub>\u2010C126\u2010X<jats:sub>2<\/jats:sub>\u2010C129, located in the minimal DNA binding region of XPA (M98\u2010F219). The zinc\u2010associated motif is essential for damaged DNA recognition. Extended X\u2010ray absorption fine structure (EXAFS) spectra collected on the zinc associated minimal DNA\u2010binding domain of XPA (ZnXPA\u2010MBD) show directly, for the first time, that the zinc is coordinated to the sulfur atoms of four cysteine residues with an average Zn\u2010S bond length of 2.34 \u00b1 0.01 \u00c5. XPA\u2010MBD was also expressed in minimal medium supplemented with cobalt nitrate to yield a blue\u2010colored protein that was primarily (&gt;95%) cobalt associated (CoXPA\u2010MBD). EXAFS spectra collected on CoXPA\u2010MBD show that the cobalt is also coordinated to the sulfur atoms of four cysteine residues with an average Co\u2010S bond length of 2.33 \u00b1 0.02 \u00c5.<\/jats:p>","DOI":"10.1002\/pro.5560070912","type":"journal-article","created":{"date-parts":[[2009,2,10]],"date-time":"2009-02-10T06:40:57Z","timestamp":1234248057000},"page":"1970-1975","source":"Crossref","is-referenced-by-count":15,"title":["Human nucleotide excision repair protein XPA: Extended X\u2010ray absorption fine\u2010structure evidence for a metal\u2010binding domain"],"prefix":"10.1002","volume":"7","author":[{"given":"Nancy J.","family":"Hess","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garry W.","family":"Buchko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shuisong","family":"Ni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brian D.","family":"Thrall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Kennedy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven D.","family":"Conradson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"F. 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