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During infection, Mrp recruit human fibrinogen (Fg) to the bacterial surface, enhancing phagocytosis resistance and promoting growth in human blood. However, Mrp exhibit a high degree of sequence diversity, clustering into four evolutionarily distinct groups. It is currently unknown whether this diversity affects the host\u2013pathogen interactions mediated by Mrp. In this study, nine Mrp sequences from the four major evolutionary groups were selected to examine the effect of sequence diversity on protein\u2013protein interactions with Fg. Negative staining transmission electron microscopy confirmed that Mrp are fibrillar proteins measuring between 45.4 and 47.3\u2009nm in length, and mass photometry confirmed the ability of Mrp to form dimers. Surface plasmon resonance was used to evaluate the affinity of each Mrp for Fg. All Mrp studied bound to Fg via Fragment D (FgD) with nanomolar affinity. Previous studies have linked the acquisition of plasminogen (Plg) by GAS Fg\u2010binding M proteins to tissue destruction and excessive stimulation of the human inflammatory response during infection. Our findings show that Mrp provide an alternative mechanism for Plg recruitment, as Plg binding by Mrp was significantly enhanced following pre\u2010incubation with Fg. These data suggest that Mrp play an important role in GAS host\u2013pathogen interactions. However, further studies are necessary to investigate the relevance of these findings in vivo.<\/jats:p>","DOI":"10.1002\/pro.70078","type":"journal-article","created":{"date-parts":[[2025,3,18]],"date-time":"2025-03-18T15:06:07Z","timestamp":1742310367000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fibrinogen\u2010binding M\u2010related proteins facilitate the recruitment of plasminogen by <i>Streptococcus pyogenes<\/i>"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9229-7694","authenticated-orcid":false,"given":"Emma\u2010Jayne","family":"Proctor","sequence":"first","affiliation":[{"name":"Molecular Horizons Research Institute and School of Science University of Wollongong  Wollongong New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hannah R.","family":"Frost","sequence":"additional","affiliation":[{"name":"Molecular Bacteriology Laboratory, European Plotkins Institute for Vaccinology (EPIV) Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhanu","family":"Mantri","sequence":"additional","affiliation":[{"name":"Molecular Horizons Research Institute and School of Science University of Wollongong  Wollongong New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandeep","family":"Satapathy","sequence":"additional","affiliation":[{"name":"Molecular Horizons Research Institute and School of Science University of Wollongong  Wollongong New South Wales Australia"},{"name":"The Broad Institute of MIT and Harvard  Cambridge Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gwena\u00eblle","family":"Botquin","sequence":"additional","affiliation":[{"name":"Molecular Bacteriology Laboratory, European Plotkins Institute for Vaccinology (EPIV) Universit\u00e9 Libre de Bruxelles  Brussels Belgium"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jody","family":"Gorman","sequence":"additional","affiliation":[{"name":"Molecular Horizons Research Institute and School of Science University of Wollongong  Wollongong New South Wales Australia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M. 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