{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T17:14:09Z","timestamp":1788455649680,"version":"build-2803163510"},"reference-count":31,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T00:00:00Z","timestamp":1761004800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"publisher","award":["2021\u2010T4\u2010AN\u201007\u2010CUP B93C22000920001"],"award-info":[{"award-number":["2021\u2010T4\u2010AN\u201007\u2010CUP B93C22000920001"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    is a Gram\u2010negative opportunistic pathogen responsible for a wide range of community\u2010associated and hospital\u2010acquired infections and a major cause of neonatal sepsis in low\u2010 and middle\u2010income countries. The pathogen's surface fimbriae, particularly the Type 3 fimbriae, are critical for bacterial adhesion, biofilm formation, and host defense evasion. MrkA, the pathogen's major Type 3 fimbrial subunit, has a structural function in fimbrial assembly, but its three\u2010dimensional structure remains to be fully elucidated. In this study, we utilized solution\u2010state nuclear magnetic resonance spectroscopy to elucidate the structure of MrkA, leveraging previously reported chemical shift assignments of a designed self\u2010complementing monomeric protein. Additionally, we confirmed the ability of monoclonal antibodies, capable of recognizing MrkA oligomers on wild\u2010type\n                    <jats:italic>Klebsiella<\/jats:italic>\n                    bacteria, to bind the recombinant MrkA protein. Our findings contribute to the evaluation of MrkA as a potential target in vaccine development against\n                    <jats:italic>Klebsiella pneumoniae<\/jats:italic>\n                    infections.\n                  <\/jats:p>","DOI":"10.1002\/pro.70343","type":"journal-article","created":{"date-parts":[[2025,10,21]],"date-time":"2025-10-21T18:27:05Z","timestamp":1761071225000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Biological and structural characterization of the Type 3 fimbrial subunit\n                    <scp>MrkA<\/scp>\n                    from\n                    <scp>\n                      <i>Klebsiella pneumoniae<\/i>\n                    <\/scp>"],"prefix":"10.1002","volume":"34","author":[{"given":"Valentina","family":"Monaci","sequence":"first","affiliation":[{"name":"Magnetic Resonance Center \u2013 CERM University of Florence  Florence Italy"},{"name":"Department of Chemistry University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Davide","family":"Oldrini","sequence":"additional","affiliation":[{"name":"GSK Vaccines Institute for Global Health (GVGH)  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gianmarco","family":"Gasperini","sequence":"additional","affiliation":[{"name":"GSK  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Banci","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center \u2013 CERM University of Florence  Florence Italy"},{"name":"Department of Chemistry University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0526-6732","authenticated-orcid":false,"given":"Francesca","family":"Cantini","sequence":"additional","affiliation":[{"name":"Magnetic Resonance Center \u2013 CERM University of Florence  Florence Italy"},{"name":"Department of Chemistry University of Florence  Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Micoli","sequence":"additional","affiliation":[{"name":"GSK Vaccines Institute for Global Health (GVGH)  Siena Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2025,10,21]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2005.04945.x"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms22084042"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/vaccines13030303"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20290"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2024.02.072"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4178-4182.2005"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/jb.170.8.3547-3553.1988"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/iai.57.1.219-224.1989"},{"key":"e_1_2_9_11_1","first-page":"353","article-title":"Automated 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monoclonal antibodies reveal distinct structural and antigenic features of MrkA","volume":"12","author":"Wang Q","year":"2017","journal-title":"PLoS One"},{"key":"e_1_2_9_29_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1003787"},{"key":"e_1_2_9_30_1","doi-asserted-by":"publisher","DOI":"10.1128\/mmbr.00090-23"},{"key":"e_1_2_9_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181958769"},{"key":"e_1_2_9_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.008610"}],"container-title":["Protein 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