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Several AGEs have been detected in human lenses, and their levels have been shown to increase with age and cataract formation. AGEs can lead to structural changes, yellow pigmentation, and cross\u2010linking of lens proteins. Studies have shown a positive correlation between AGEs levels and lens age, stiffness, and cataracts. Recent research suggests that DJ\u20101 can inhibit the accumulation of AGEs in cellular proteins by reversing the early glycation steps. Our study found that DJ\u20101 is present in epithelial cells and the outer cortex of the human lens. DJ\u20101 is catalytically active in human lenses, and its ability to metabolize methylglyoxal (MGO) into D\u2010lactate diminishes as the lens ages. The formation of MGH\u20101 from MGO was promoted in lens proteins treated with the DJ\u20101 inhibitor. Recombinant DJ\u20101 prevents \u03b1\u2010dicarbonyl\u2010mediated cross\u2010linking and AGE accumulation in human \u03b1B\u2010crystallin. DJ\u20101 prevents glyoxal\u2010mediated cell death and AGE accumulation in human lens epithelial cells. Taken together, our results suggest that DJ\u20101 in the human lens hinders AGE accumulation. Enhancing its activity using pharmacological agents can potentially delay or prevent the onset of presbyopia and cataracts.<\/jats:p>","DOI":"10.1002\/pro.70375","type":"journal-article","created":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:57:16Z","timestamp":1763020636000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["<scp>DJ<\/scp>\n                    \u20101 in human lenses: Potential role in preventing advanced glycation endproduct formation"],"prefix":"10.1002","volume":"34","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0304-4255","authenticated-orcid":false,"given":"Sandip K.","family":"Nandi","sequence":"first","affiliation":[{"name":"Department of Chemistry BITS Pilani, K. K. Birla Goa Campus  Goa India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rooban B.","family":"Nahomi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shivani","family":"Rathee","sequence":"additional","affiliation":[{"name":"School of Health Sciences and Technology University of Petroleum and Energy Studies  Dehradun Uttarakhand India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neeti","family":"Gupta","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjeev Kumar","family":"Mittal","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology All India Institute of Medical Sciences  Rishikesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ram H.","family":"Nagaraj","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, School of Medicine University of Colorado  Aurora Colorado USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2025,11,12]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.RA119.011237"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.82.21.7193"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1077209"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2017.01.018"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2015.07.014"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1444-0938.2010.00516.x"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2015.09.005"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddq113"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pep.2022.106092"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4835(77)90139-7"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.699850"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M800580-MCP200"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1802901115"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.3c00028"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.535112"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1006\/exer.1994.1135"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4644(19990301)72:3<435::AID-JCB12>3.0.CO;2-H"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0409635102"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.freeradbiomed.2009.08.003"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.3858\/emm.2012.44.2.012"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/467130"},{"key":"e_1_2_11_23_1","first-page":"569","article-title":"Accommodation and presbyopia in the human eye. 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