{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T17:41:04Z","timestamp":1789839664566,"version":"4.0.1"},"reference-count":36,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T00:00:00Z","timestamp":1777248000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["2231900"],"award-info":[{"award-number":["2231900"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["IIS2211598"],"award-info":[{"award-number":["IIS2211598"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI2433490"],"award-info":[{"award-number":["DBI2433490"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI2146026"],"award-info":[{"award-number":["DBI2146026"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["DBI2422620"],"award-info":[{"award-number":["DBI2422620"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000001","name":"National Science Foundation","doi-asserted-by":"publisher","award":["MRI 1531991"],"award-info":[{"award-number":["MRI 1531991"]}],"id":[{"id":"10.13039\/100000001","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Ferritinophagy is a central pathway in cellular iron homeostasis, yet the molecular basis by which the selective autophagy receptor NCOA4 recognizes and engages ferritin remains poorly defined, largely due to the long\u2010standing inability of working with a soluble form of the full\u2010length human NCOA4 (NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    ). Here, we present an integrated biochemical and biophysical analysis of NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    and its interaction with human ferritin, complemented by structure\u2010guided modeling. We show that NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    is predominantly intrinsically disordered and exists in a dynamic monomer\u2013dimer equilibrium in solution, yet forms a stable, nanomolar\u2010affinity complex with ferritin. Using crosslinking mass spectrometry, together with integrative modeling informed by low\u2010resolution structural context, we demonstrate that NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    engages ferritin through a multivalent, distributed interface that is not confined to a short motif, in contrast to prior fragment\u2010based models. Multiple interaction hotspots are identified on ferritin helices B and D, while NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    employs both central and terminal regions to wrap around the ferritin nanocage, enabling avidity\u2010driven recognition. We further show that NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    coordinates a redox\u2010sensitive [4Fe\u20134S] cluster, introducing a chemically defined feature that may contribute to its conformational plasticity and regulation. Together, these findings establish NCOA4\n                    <jats:sup>FL<\/jats:sup>\n                    as a flexible, multivalent ferritin receptor and define the recognition logic by which ferritin assemblies are selectively recognized and targeted for lysosomal degradation during ferritinophagy.\n                  <\/jats:p>","DOI":"10.1002\/pro.70589","type":"journal-article","created":{"date-parts":[[2026,4,27]],"date-time":"2026-04-27T13:48:03Z","timestamp":1777297683000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Multivalent recognition of ferritin by full\u2010length\n                    <scp>NCOA4<\/scp>\n                    enables robust ferritinophagy"],"prefix":"10.1002","volume":"35","author":[{"given":"Ayush K.","family":"Srivastava","sequence":"first","affiliation":[{"name":"Department of Chemistry State University of New York  Potsdam New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Genki","family":"Terashi","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Purdue University  West Lafayette Indiana USA"},{"name":"Department of Computer Science Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosa","family":"Viner","sequence":"additional","affiliation":[{"name":"Thermo Fisher Scientific  San Jose California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Audrey","family":"Kishishita","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7465-6964","authenticated-orcid":false,"given":"Arun P.","family":"Wiita","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine University of California San Francisco  San Francisco California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anitha","family":"Rajendran","sequence":"additional","affiliation":[{"name":"Department of Chemistry State University of New York  Potsdam New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yeonni","family":"Zoo","sequence":"additional","affiliation":[{"name":"Department of Physics Villanova University  Villanova Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Georgia C.","family":"Papaefthymiou","sequence":"additional","affiliation":[{"name":"Department of Physics Villanova University  Villanova Pennsylvania USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daisuke","family":"Kihara","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Purdue University  West Lafayette Indiana USA"},{"name":"Department of Computer Science Purdue University  West Lafayette Indiana USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8557-1827","authenticated-orcid":false,"given":"Fadi","family":"Bou\u2010Abdallah","sequence":"additional","affiliation":[{"name":"Department of Chemistry State University of New York  Potsdam New York USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,4,27]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-024-07487-w"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms26010206"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2010.03.021"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijbiomac.2024.135044"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.5104"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00775-016-1336-0"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.3390\/inorganics11120450"},{"key":"e_1_2_9_9_1","first-page":"197","volume-title":"Methods in enzymology","author":"Freibert 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algorithms for rapid unsupervised cryo\u2010EM structure determination","volume":"14","author":"Punjani A","year":"2017","journal-title":"Nat Methods"},{"issue":"2","key":"e_1_2_9_27_1","doi-asserted-by":"crossref","first-page":"216","DOI":"10.1016\/j.jsb.2015.08.008","article-title":"CTFFIND4: fast and accurate defocus estimation from electron micrographs","volume":"192","author":"Rohou A","year":"2015","journal-title":"J Struct 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improved cryo\u2010electron microscopy","volume":"14","author":"Zheng SQ","year":"2017","journal-title":"Nat Methods"}],"container-title":["Protein 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