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Intrinsically disordered proteins (IDPs) are ubiquitous in viral proteomes, where conformational plasticity not only enables functional diversity but also permits aberrant self\u2010assembly. Members of the\n                    <jats:italic>Paramyxoviridae<\/jats:italic>\n                    family, including henipaviruses such as the Nipah and Hendra viruses (two biosafety level\u20104 pathogens), encode the P, V, and W proteins that share a common intrinsically disordered N\u2010terminal domain (NTD). V and W are virulence factors that undergo fibrillation. Here, we explored the aggregation landscape of seven homologous PNT1 subdomains from the NTD that harbors a conserved cryptic amyloidogenic region (CAR). By integrating Taylor dispersion analysis (TDA), Raman spectroscopy, and all\u2010atom molecular dynamics (MD) simulations, we unraveled both early assembly kinetics and structural outcomes. Despite the conserved CAR motif, the PNT1 homologs exhibit strikingly divergent aggregation behaviors. TDA revealed distinct oligomerization pathways with variations in nucleation, growth kinetics, and monomer recruitment, indicating virus\u2010specific pathways. Raman spectroscopy unveiled substantial variations in \u03b2\u2010sheet content, side\u2010chain packing, and aromatic residue environments, while MD simulations showed that conformational heterogeneity in the CAR flanking regions modulates hydrogen\u2010bond networks and structural stability. These findings indicate that the sequence context beyond a conserved amyloidogenic core governs the aggregation landscape of\n                    <jats:italic>Henipavirus<\/jats:italic>\n                    P, V, and W proteins. Evolutionary diversification modulates conformational heterogeneity and assembly pathways, giving rise to distinct structural outcomes. This work sheds light on the molecular grammar underlying viral protein fibrillation with potential implications for viral pathogenicity.\n                  <\/jats:p>","DOI":"10.1002\/pro.70758","type":"journal-article","created":{"date-parts":[[2026,8,11]],"date-time":"2026-08-11T11:53:20Z","timestamp":1786449200000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Sequence\u2010encoded determinants drive distinct early aggregation pathways and different structural outcomes in\n                    <scp>PNT1<\/scp>\n                    fibrils across\n                    <i>Henipavirus<\/i>\n                    members"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-8219-2817","authenticated-orcid":false,"given":"Harshita","family":"Sawdekar","sequence":"first","affiliation":[{"name":"Aix\u2010Marseille Universit\u00e9, CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques (AFMB), UMR 7257  Marseille France"},{"name":"IBMM, University of Montpellier, CNRS, ENSCM  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1223-4501","authenticated-orcid":false,"given":"Julien","family":"Mignon","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille Universit\u00e9, CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques (AFMB), UMR 7257  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank","family":"Gondelaud","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille Universit\u00e9, CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques (AFMB), UMR 7257  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anuja","family":"Walimbe","sequence":"additional","affiliation":[{"name":"Centre for Protein Science, Design, and Engineering Indian Institute of Science Education and Research (IISER)  Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1242-9958","authenticated-orcid":false,"given":"Samrat","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Centre for Protein Science, Design, and Engineering Indian Institute of Science Education and Research (IISER)  Mohali Punjab India"},{"name":"Department of Chemical Sciences Indian Institute of Science Education and Research (IISER)  Mohali Punjab India"},{"name":"Department of Biological Sciences Indian Institute of Science Education and Research (IISER)  Mohali Punjab India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4209-1337","authenticated-orcid":false,"given":"Joseph","family":"Chamieh","sequence":"additional","affiliation":[{"name":"IBMM, University of Montpellier, CNRS, ENSCM  Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6829-6771","authenticated-orcid":false,"given":"Sonia","family":"Longhi","sequence":"additional","affiliation":[{"name":"Aix\u2010Marseille Universit\u00e9, CNRS, Architecture et Fonction des Macromol\u00e9cules Biologiques (AFMB), UMR 7257  Marseille France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2026,8,11]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.softx.2015.06.001"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313134200"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1063\/5.0020514"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1021\/jz3019786"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.4918"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.3390\/v13122465"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2022.167919"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1063\/1.2408420"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.202200192"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.analchem.1c00527"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-021-02797-5"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-20164-1_12"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jcim.0c01360"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.4331"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.70085"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/advs.202523465"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/pro.70068"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2023.102706"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0011684"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.4776"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.saa.2024.124377"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1096-987X(199709)18:12<1463::AID-JCC4>3.0.CO;2-H"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1021\/ct5010406"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.4067"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/0263-7855(96)00018-5"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.jpcb.4c04100"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20945"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.3201\/eid3103.241134"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2023.2273684"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009740"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1039\/C8RA04491K"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2023.1167085"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja00438a057"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-017-2556-3"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1039\/D4SM01392A"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1021\/ja0356176"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41592-022-01488-1"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi00401a015"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00547608"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24010399"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jmb.2023.167995"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.17239"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms23020923"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-022-00670-9"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku316"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.chroma.2024.465325"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.4335"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.3390\/biom11091324"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.78.11.5633-5641.2004"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/22.22.4673"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1128\/jvi.00770-25"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1002\/jrs.1323"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr500288y"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcc.20291"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1080\/08927028808080941"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1021\/cr4005692"}],"container-title":["Protein 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