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Here, we present a simple, modular approach to developing multispecific, multivalent Abs based on a Fab\u2010phage library with a single light chain. Using this library, we created three Abs targeting unique sites on programmed death\u2010ligand 1 (PD\u2010L1) and another antibody targeting CD30. Biophysical and cellular characterization of these Abs demonstrated their functional equivalence to clinically relevant Abs targeting PD\u2010L1 or CD30. We then combined these paratopes into a series of bispecific, tetravalent, triparatopic Abs that retained the functionality of the parental Abs. Structural analysis of each of the Abs in complex with their cognate antigens demonstrated the adaptability of the common light chain to form diverse paratopes with an array of distinct heavy chains.<\/jats:p>","DOI":"10.1002\/pro.70797","type":"journal-article","created":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T10:16:12Z","timestamp":1789726572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Strategy for modular assembly of tetravalent, multispecific antibodies"],"prefix":"10.1002","volume":"35","author":[{"given":"Evan","family":"Mallette","sequence":"first","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"},{"name":"Department of Chemistry and Biochemistry Wilfrid Laurier University  Waterloo Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9594-4642","authenticated-orcid":false,"given":"Levi L.","family":"Blazer","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"},{"name":"Aarvik Therapeutics  Hayward California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4719-3842","authenticated-orcid":false,"given":"Craig A.","family":"Hokanson","sequence":"additional","affiliation":[{"name":"Simisco Biosciences  Hayward California USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao","family":"Chen","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia Garcia","family":"Perez","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alevtina","family":"Pavlenco","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lynda","family":"Ploder","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander U.","family":"Singer","sequence":"additional","affiliation":[{"name":"The Anvil Institute of Systems Biologics  Toronto Ontario Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D. 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