{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:08:21Z","timestamp":1790856501125,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:00:00Z","timestamp":1790812800000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["IR0000010"],"award-info":[{"award-number":["IR0000010"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000780","name":"European Commission","doi-asserted-by":"publisher","award":["CN00000041"],"award-info":[{"award-number":["CN00000041"]}],"id":[{"id":"10.13039\/501100000780","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Protein Science"],"published-print":{"date-parts":[[2026,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Electrostatic interactions play a crucial role in protein structure, stability, and function. We present Bluues2, a significantly enhanced web server for calculating electrostatic properties of proteins using the Generalized Born model. The new version features improved accuracy through refined GB radius calculations using NanoShaper for molecular surface generation, extended functionality for analyzing protein\u2010protein and protein\u2010ligand complexes including calculation of binding free energies and electrostatic complementarity, enhanced interface with integrated Mol* structure viewer, and compatibility with the DRMAAtic framework for efficient job management. Bluues2 provides comprehensive electrostatic analyses including pKa predictions, surface potentials, solvation free energies, and complex interface properties in a user\u2010friendly format accessible to both experts and non\u2010specialists.<\/jats:p>","DOI":"10.1002\/pro.70815","type":"journal-article","created":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T11:37:09Z","timestamp":1790854629000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Bluues2: An enhanced web server for fast and accurate electrostatic analysis of proteins and protein complexes"],"prefix":"10.1002","volume":"35","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8210-2390","authenticated-orcid":false,"given":"Damiano","family":"Piovesan","sequence":"first","affiliation":[{"name":"Department of Biomedical Sciences University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7284-6505","authenticated-orcid":false,"given":"Nima","family":"Rasekh","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences University of Padua  Padua Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5780-9949","authenticated-orcid":false,"given":"Miguel Angel","family":"Soler","sequence":"additional","affiliation":[{"name":"Department of Molecular Sciences and Nanosystems Ca' Foscari University of Venice  Venice Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2480-7151","authenticated-orcid":false,"given":"Walter","family":"Rocchia","sequence":"additional","affiliation":[{"name":"CONCEPT Lab Istituto Italiano di Tecnologia  Genoa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9353-5423","authenticated-orcid":false,"given":"Gennaro","family":"Esposito","sequence":"additional","affiliation":[{"name":"Istituto Nazionale Biostrutture e Biosistemi  Rome Italy"},{"name":"Science and Math Division New York University at Abu Dhabi  Abu Dhabi United Arab Emirates"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9862-250X","authenticated-orcid":false,"given":"Federico","family":"Fogolari","sequence":"additional","affiliation":[{"name":"Department of Mathematics, Computer Science and Physics University of Udine  Udine Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4525-7793","authenticated-orcid":false,"given":"Silvio C. 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