{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:55:33Z","timestamp":1790571333174,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","license":[{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"},{"start":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T00:00:00Z","timestamp":1790467200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["82373196"],"award-info":[{"award-number":["82373196"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Precision Radiation Oncology"],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background and purpose<\/jats:title>\n                    <jats:p>Head and neck squamous cell carcinoma (HNSCC) exhibits heterogeneous responses to radiotherapy; however, the underlying multi\u2010omics regulatory networks remain poorly characterized. This study elucidated the distinct post\u2010translational modification (PTM) landscapes induced by X\u2010ray and carbon\u2010ion irradiation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Using integrated proteomics, phosphoproteomics, and acetylomics, we systematically profiled PTMs in HSC\u20103 cells at 30 min post\u2010irradiation with X\u2010ray (8 Gy) or carbon ions (4 GyE). Computational analyses included functional annotation, kinase activity prediction, and PTM crosstalk mapping.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Carbon ions induced 21% more phosphosites (2,781 vs. 2,301) and 61% more acetyl sites (3,324 vs. 2,070) than X\u2010ray, demonstrating broader PTM reprogramming. Notably, carbon ion\u2010induced histone hypoacetylation (H2AZ1K8\/K5, H3C1K28\/K24, and H4C1K92) and hyperphosphorylation (H1\u201010S2 and H3C1T119) potentially alter chromatin accessibility. Functional enrichment analyses revealed the activation of the non\u2010homologous end\u2010joining (NHEJ) pathway and coordinated upregulation of antigen\u2010presenting machinery (such as CD274, TAP2, HLA\u2010A, and PSMB8) through PTM remodeling. Distinct PTM signatures in RNA splicing factors implicate alternative splicing in radiation response. Kinase activity profiling revealed ATM hyperactivation and CDK suppression in the carbon ion group.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Carbon ion irradiation drives distinct PTM\u2010mediated immunomodulatory and DNA damage\u2010response networks, providing a mechanistic rationale for combining carbon ion radiotherapy with immunotherapy. Future studies should validate the PTM\u2010associated targets to improve HNSCC treatment.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pro6.70097","type":"journal-article","created":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T04:11:03Z","timestamp":1790568663000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Integrated multi\u2010omics analysis of post\u2010translational modifications in head and neck squamous cell carcinoma: elucidating x\u2010ray and carbon ion irradiation effects"],"prefix":"10.1002","author":[{"ORCID":"https:\/\/orcid.org\/0009-0002-3893-8638","authenticated-orcid":false,"given":"Fan\u2010Tong","family":"Xia","sequence":"first","affiliation":[{"name":"Radiation Oncology Center Chongqing University Cancer Hospital College of Medicine Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi\u2010Yun","family":"Liao","sequence":"additional","affiliation":[{"name":"Radiation Oncology Center Chongqing University Cancer Hospital College of Medicine Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Si\u2010Qi","family":"Cai","sequence":"additional","affiliation":[{"name":"Radiation Oncology Center Chongqing University Cancer Hospital College of Medicine Chongqing University  Chongqing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li\u2010Rong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Lanzhou Ion Therapy Co. 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