{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T05:38:16Z","timestamp":1767850696045,"version":"3.49.0"},"reference-count":46,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2002,3,4]],"date-time":"2002-03-04T00:00:00Z","timestamp":1015200000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2002,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Ecto\u2010diadenosine polyphosphates are ubiquitous compounds with several physiological roles. Ecto\u2010diadenosine polyphosphates hydrolase control their actions by degrading and terminating their signaling. The present work deals with the identification and partial characterization of ecto\u2010diadenosine polyphosphates hydrolase on human prostasomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Reverse\u2010phase and paired\u2010ion HPLC techniques have been used.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Prostasomes have an ecto\u2010diadenosine polyphosphates hydrolase that leads to the degradation of several diadenosine compounds. Kinetic parameters of the enzyme show that diadenosine tetraphosphate is the preferred substrate that is further metabolized by the prostasome\u2010ecto\u2010nucleotidases to adenosine. The ecto\u2010enzyme is bound to the prostasome\u2010membranes through a GPI\u2010anchor and is activated by physiological concentration of Ca<jats:sup>+2<\/jats:sup>, Mg<jats:sup>+2<\/jats:sup>, and Mn<jats:sup>+2<\/jats:sup>. Its optimum pH is also in the slightly alkaline physiological range. Human spermatozoa do not possess this hydrolytic activity, but they can acquire it after fusion with prostasomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The existence of an enzyme capable of degrading diadenosine compounds and can be transferred to human spermatozoa suggests new physiological implications for the role of prostasomes in fertilization. <jats:italic>Prostate<\/jats:italic> 51: 1\u20139, 2002. \u00a9 2002 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.10062","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T23:13:41Z","timestamp":1030317221000},"page":"1-9","source":"Crossref","is-referenced-by-count":14,"title":["Ecto\u2010diadenosine polyphosphates hydrolase activity on human 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