{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,2]],"date-time":"2025-12-02T06:04:55Z","timestamp":1764655495330},"reference-count":17,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2003,5,7]],"date-time":"2003-05-07T00:00:00Z","timestamp":1052265600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2003,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The database of human Expressed Sequence Tags was previously used to identify <jats:italic>PRAC<\/jats:italic> (Prostate 47:125\u2013131, 2001), a novel gene specifically expressed in human prostate, prostate cancer, rectum, and distal colon. In this report, we have identified <jats:italic>PRAC2<\/jats:italic>, another gene with a similar expression pattern that is located adjacent to the original <jats:italic>PRAC<\/jats:italic> gene on chromosome 17q21.3.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Using a computer\u2010based analysis, a cluster of sequence homologous ESTs was identified that is mainly derived from human prostate cDNA libraries. The tissue specificity was examined by multiple tissue RNA dot blots and RT\u2010PCR. The <jats:italic>PRAC2<\/jats:italic> transcript and protein were identified using Northern blot analysis, RACE\u2010PCR, primer extension, and Western blots.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p><jats:italic>PRAC2<\/jats:italic> encodes a 564 nucleotide RNA found in prostate, rectum, distal colon, and testis. Weak expression was also found in placenta, peripheral blood leukocytes, skin, and in two prostate cancer cell lines: LNCaP and PC\u20103. The transcript seems to encode a 10.5\u2010kDa nuclear protein. The <jats:italic>PRAC2<\/jats:italic> gene is located on chromosome 17 at position 17q21, between the <jats:italic>Hoxb\u201013<\/jats:italic> gene and the recently discovered <jats:italic>PRAC<\/jats:italic> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Because of the higher expression of PRAC2 in prostate and its proximity to <jats:italic>Hoxb\u201013<\/jats:italic>, PRAC2 may have a function in prostate growth and development. Prostate 56: 123\u2013130, 2003. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.10185","type":"journal-article","created":{"date-parts":[[2003,5,9]],"date-time":"2003-05-09T10:08:34Z","timestamp":1052474914000},"page":"123-130","source":"Crossref","is-referenced-by-count":23,"title":["<i>PRAC2<\/i>: A new gene expressed in human prostate and prostate cancer"],"prefix":"10.1002","volume":"56","author":[{"given":"P\u00e4r","family":"Olsson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Motegi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tapan K.","family":"Bera","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Byungkook","family":"Lee","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ira","family":"Pastan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,5,7]]},"reference":[{"key":"e_1_2_1_2_2","volume-title":"Prostate cancer: What every man and his family needs to know","author":"Bostwick DG","year":"1999"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.1.300"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.160270597"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1102"},{"key":"e_1_2_1_6_2","first-page":"4752","article-title":"XAGE\u20101, a new gene that is frequently expressed in Ewing's sarcoma","volume":"60","author":"Liu XF","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052713699"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.95.18.10757"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1055"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.16.9287"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2836(92)90320-J"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.08.110192.001213"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.9.1678"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.8.2475"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(19991101)41:3<203::AID-PROS8>3.0.CO;2-J"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0142(19990501)85:9<2017::AID-CNCR20>3.0.CO;2-V"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0215(19990219)84:1<19::AID-IJC4>3.0.CO;2-S"},{"key":"e_1_2_1_18_2","first-page":"1241","article-title":"Localization of potential tumor suppressor loci to a\u2009<\u20092 Mb region on chromosome 17q in human prostate cancer","volume":"11","author":"Gao X","year":"1995","journal-title":"Oncogene"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.10185","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.10185","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T14:22:18Z","timestamp":1700230938000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.10185"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,5,7]]},"references-count":17,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2003,7]]}},"alternative-id":["10.1002\/pros.10185"],"URL":"https:\/\/doi.org\/10.1002\/pros.10185","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,5,7]]}}}