{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T13:14:05Z","timestamp":1762175645935},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2003,10,30]],"date-time":"2003-10-30T00:00:00Z","timestamp":1067472000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2004,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The serine\/threonine kinase Raf\u20101 is a major regulator of the mitogen activated protein kinase (MAPK) pathway, which has been associated with the progression of prostate cancer to the more advanced and androgen\u2010independent disease. Cdc25A phosphatase has been implicated in the regulation of Raf\u20101 and the MAPK pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We used a novel and potent Cdc25A inhibitor, 2,3\u2010bis\u2010[2\u2010hydroxyethylsulfonyl]\u2010[1,4] naphthoquinone (NSC 95397), and its congener (2\u2010mercaptoethanol)\u20103\u2010methyl\u20101, 4\u2010naphthoquinone (NSC 672121) to study the role of Cdc25A on the MAPK pathway in human prostate cancer cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We found Raf\u20101 physically interacted with Cdc25A in PC\u20103 and LNCap cells and inhibitors of Cdc25A induced both extracellular signal\u2010regulated kinase (Erk) activation and Raf\u20101 tyrosine phosphorylation. NSC 95397 attenuated Cdc25A and Raf\u20101 interactions due to accelerated degradation of Cdc25A, which was mediated by proteasome degradation. The MAPK kinase (MEK) inhibitor U0126 completely inhibited Erk activation by NSC 95397 and NSC 672121.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results indicate Cdc25A phosphatase regulates Raf\u20101\/MEK\/Erk kinase activation in human prostate cancer cells. \u00a9 2003 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.10292","type":"journal-article","created":{"date-parts":[[2003,12,10]],"date-time":"2003-12-10T10:00:35Z","timestamp":1071050435000},"page":"95-102","source":"Crossref","is-referenced-by-count":24,"title":["Activation of the Raf\u20101\/MEK\/Erk kinase pathway by a novel Cdc25 inhibitor in human prostate cancer cells"],"prefix":"10.1002","volume":"58","author":[{"given":"Kaoru","family":"Nemoto","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andreas","family":"Vogt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tetsuya","family":"Oguri","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John S.","family":"Lazo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2003,10,30]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00001622-199801000-00014"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1206121"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf567"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7667636"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI9174"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd963"},{"key":"e_1_2_1_8_2","first-page":"278","article-title":"Activation of mitogen\u2010activation protein kinase associated with prostate cancer progression","volume":"59","author":"Gioeli D","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1053\/hupa.2002.32227"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9861(02)00244-8"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.9.9.1046"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.7.4819"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M004583200"},{"key":"e_1_2_1_14_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111598200"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100078200"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1124\/mol.61.4.720"},{"key":"e_1_2_1_17_2","first-page":"1317","article-title":"Cdc25 inhibition and cell cycle arrest by a synthetic thioalkyl vitamin K analogue","volume":"60","author":"Tamura K","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1994.tb06750.x"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.19.9.6183"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-7258(00)00085-1"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.13.11.7170"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202362"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.288.5470.1425"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/embo-reports\/kvd018"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/35071124"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf357"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90401-8"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.21.14.4818-4828.2001"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.26.18659"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/35090585"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4652(200006)183:3<338::AID-JCP6>3.0.CO;2-X"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.10292","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.10292","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,17]],"date-time":"2023-11-17T16:20:42Z","timestamp":1700238042000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.10292"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2003,10,30]]},"references-count":30,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2004,1]]}},"alternative-id":["10.1002\/pros.10292"],"URL":"https:\/\/doi.org\/10.1002\/pros.10292","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2003,10,30]]}}}