{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T23:44:52Z","timestamp":1784763892525,"version":"3.55.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2002,4,4]],"date-time":"2002-04-04T00:00:00Z","timestamp":1017878400000},"content-version":"vor","delay-in-days":246,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2001,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The canine prostate has often been proposed as a model for abnormal growth of the human gland. Hyperplasia of the prostate is common in aging men and has been estimated to be present in 100% of old intact dogs. While prostatic carcinoma is common in older men, it appears to be rare in dogs and unlike the disease in humans, it occurs with relatively high frequency in castrated animals. Since basal cells are thought to be key participants in normal and abnormal growth of the human gland, we used immunohistochemistry to investigate the role that they may play in canine prostatic development, the evolution of hyperplasia and carcinoma, and the effects of sex hormones on these cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Prostate specimens were obtained at autopsy from seven sexually immature dogs, autopsy and biopsy samples from 14 sexually mature intact animals, from four castrates, and from19 dogs with prostatic carcinoma. In addition, we also studied the prostates from two intact dogs treated with 5\u03b1\u2010dihydrotestosterone (DHT) for 6 months and two castrated dogs that were subsequently treated with 5\u03b1\u2010androstane\u20103\u03b1 diol and estradiol\u201017\u03b1, as well as specimens from two sexually ablated animals given DHT for 2 weeks. All specimens were immunostained for high molecular weight cytokeratin (HMC), pancytokeratin, androgen receptor (AR), and the proliferative marker KI\u201067.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We find that basal cells are the major proliferative cell type in the neonatal and adult canine prostate and that the expression of HMC staining, which defines these cells, may be regulated by androgens. In the adult gland, ductal basal cells formed a contiguous layer, whereas those lining acini were discontinuous. Populations of both basal cell types were variably AR positive, but while HMC immunostaining was abolished in acinar cells following long\u2010term castration, staining remained in ductal cell counterparts. Paralleling the histological development of hyperplasia, the acinar basal cell population increased with age and were the major cell type that expressed<jats:italic>KI<\/jats:italic>\u201067. In contrast, ductal basal cell populations did not expand in the prostates of older dogs and were seldom positively stained for<jats:italic>KI<\/jats:italic>\u201067. The numbers of HMC and KI\u201067\u2010stained acinar basal cells were dramatically increased in the prostates of intact dogs treated with DHT when compared with glands of untreated controls. This was not the case with ductal basal cells. Androgens given alone or together with estrogen to castrated dogs induced widespread HMC and<jats:italic>KI<\/jats:italic>\u201067 immunostaining in both populations of basal cells. In addition, our results indicate that the majority of canine prostatic carcinomas likely arise exclusively from ductal epithelium. Only one of the 19 cases of carcinoma contained cells that expressed AR, which suggests that androgens may not be required for the initiation or progression of these cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings indicate that two biologically distinct populations of basal cells may exist in the canine prostate. In this regard, the age\u2010related expansion of proliferating acinar basal cell populations, probably mediated by sex steroids, is a key factor in the pathogenesis of canine prostatic hyperplasia. Additionally, we find that prostatic carcinoma in the dog likely arises from ductal cells. Taken together, these findings may indicate that canine acinar basal cells and ductal epithelium have separate susceptibilities to factors that promote hyperplastic or neoplastic development. Prostate 48:210\u2013224, 2001. \u00a9 2001 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.1100","type":"journal-article","created":{"date-parts":[[2002,8,25]],"date-time":"2002-08-25T21:50:36Z","timestamp":1030312236000},"page":"210-224","source":"Crossref","is-referenced-by-count":57,"title":["Role of canine basal cells in postnatal prostatic development, induction of hyperplasia, and sex hormone\u2010stimulated growth; and the ductal origin of carcinoma*"],"prefix":"10.1002","volume":"48","author":[{"given":"Irwin","family":"Leav","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly H.","family":"Schelling","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason Y.","family":"Adams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederick B.","family":"Merk","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph","family":"Alroy","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2002,4,4]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"109","volume-title":"Benign prostatic hyperplasia","author":"Strandberg JD","year":"1985"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990090406"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990090308"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/030098589002700507"},{"key":"e_1_2_1_6_2","first-page":"791","article-title":"Diseases of the canine prostate gland","volume":"17","author":"Dorfman M","year":"1995","journal-title":"Compend Contin Educ Pract Vet"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-114-2-511"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)49698-4"},{"key":"e_1_2_1_9_2","first-page":"340","article-title":"Origin and evolution of benign prostatic enlargement","volume":"15","author":"McNeal JE","year":"1978","journal-title":"Invest Urol"},{"key":"e_1_2_1_10_2","doi-asserted-by":"crossref","first-page":"348","DOI":"10.1002\/j.1939-4640.1991.tb00271.x","article-title":"The origin and development of benign prostatic hyperplasia. 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