{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T17:46:31Z","timestamp":1791135991505,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2004,2,2]],"date-time":"2004-02-02T00:00:00Z","timestamp":1075680000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2004,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Elevated expression of interleukin\u20106 (IL\u20106) is implicated in the progression of hormone refractory prostate cancer. Previous studies demonstrated that IL\u20106 promotes androgen\u2010independent growth of prostate cancer cells. In this study, the effect of IL\u20106 on apoptosis induced by androgen deprivation was investigated.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>The effect of IL\u20106 on apoptosis induced by androgen deprivation in LNCaP cells was examined by cell death ELISA and Western blot using cleaved poly (ADP\u2010ribose) polymerase (PARP) and caspase\u20109, as well as Bcl\u2010x<jats:sub>L<\/jats:sub> and phosphorylated Bad. The Stat3 in IL\u20106\u2010mediated anti\u2010apoptosis in prostate cancer cells was examined using either dominant\u2010negative or constitutively activated Stat3 mutants.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Overexpression of IL\u20106 renders androgen sensitive LNCaP human prostate cancer cells more resistant to apoptosis induced by androgen deprivation. LNCaP cells undergo apoptosis after 72 hr of androgen deprivation, an outcome is largely absent in clones overexpressing IL\u20106 as measured by cell death ELISA and chromatin degradation assays. IL\u20106 over\u2010expressing cells resulted in a significant decrease in the expression of cleaved PARP and cleaved caspase\u20109 as well as an increase in the expression of Bcl\u2010x<jats:sub>L<\/jats:sub> and phosphorylated Bad. Addition of IL\u20106 antibody completely abolished the anti\u2010apoptotic activity of IL\u20106. This protective effect of IL\u20106 was reversed by the expression of a dominant\u2010negative Stat3 mutant, Stat3F. Furthermore, ectopic expression of a constitutively active Stat3 antagonized androgen deprivation\u2010induced cell death of LNCaP cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>These results indicate that IL\u20106 protects androgen sensitive LNCaP cells from apoptosis induced by androgen deprivation, and Stat3 activation play an important role in IL\u20106\u2010mediated anti\u2010apoptosis in prostate cancer cells. \u00a9 2004 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.20045","type":"journal-article","created":{"date-parts":[[2004,5,28]],"date-time":"2004-05-28T17:00:48Z","timestamp":1085763648000},"page":"178-186","source":"Crossref","is-referenced-by-count":71,"title":["RETRACTED: Interleukin\u20106 protects LNCaP cells from apoptosis induced by androgen deprivation through the Stat3 pathway"],"prefix":"10.1002","volume":"60","author":[{"given":"Soo 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