{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T13:53:56Z","timestamp":1777989236760,"version":"3.51.4"},"reference-count":45,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,12,12]],"date-time":"2006-12-12T00:00:00Z","timestamp":1165881600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2007,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>BXL\u2010628 is a calcitriol analog shown to decrease prostate growth in preclinical and clinical studies. BPH symptoms are generated not only by prostate overgrowth but also by bladder overactivity, resulting from an increased RhoA\/Rho\u2010kinase signaling. Because bladder smooth muscle cells express VDR, we studied effects of BXL\u2010628 on this pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>RhoA and Rho\u2010kinase gene expression and functional activity were studied in rat and human bladder smooth muscle by real\u2010time RT\u2010PCR, immuno\u2010kinase assays, western blot analysis, confocal microscopy, in vitro contractility, and cell migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>In bladder smooth muscle, carbachol responsiveness was delayed and Rho\u2010kinase activity reduced by BXL\u2010628 treatment because of impaired RhoA membrane translocation and activation. Accordingly, RhoA\u2010mediated biological functions, such as cell migration and cytoskeleton remodeling were also inhibited by BXL\u2010628.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>BXL\u2010628 inhibits RhoA\/Rho\u2010kinase signaling, a calcium sensitizing pathway, suggesting its possible clinical use in the treatment of altered bladder contractility often associated with BPH\u2010induced lower urinary tract symptoms. Prostate 67:234\u2013247, 2007. \u00a9 2006 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.20463","type":"journal-article","created":{"date-parts":[[2006,12,12]],"date-time":"2006-12-12T22:18:12Z","timestamp":1165961892000},"page":"234-247","source":"Crossref","is-referenced-by-count":66,"title":["BXL\u2010628, a vitamin D receptor agonist effective in benign prostatic hyperplasia treatment, prevents RhoA activation and inhibits RhoA\/Rho kinase signaling in rat and human bladder"],"prefix":"10.1002","volume":"67","author":[{"given":"Annamaria","family":"Morelli","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linda","family":"Vignozzi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sandra","family":"Filippi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gabriella 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