{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T22:54:00Z","timestamp":1790204040303,"version":"4.1.0"},"reference-count":68,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2008,1,14]],"date-time":"2008-01-14T00:00:00Z","timestamp":1200268800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>MT1\u2010MMP is a metalloproteinase involved in prostate cancer metastasis. The IGF\u20101R is a tyrosine kinase receptor involved with tumor progression and metastasis. The purpose of this investigation was to examine MT1\u2010MMP and IGF\u20101R expression and localization in prostate cancer tissues and explore the role of IGF\u20101R in regulating MT1\u2010MMP in prostate cancer cell lines.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Immunohistochemistry was utilized to study MT1\u2010MMP and IGF\u20101R expression in human prostate tissues. IGF\u20101R regulation of MT1\u2010MMP expression was determined by gene promoter analysis, quantitative RT\u2010PCR and Western blot analysis following pharmacological inhibition of the receptor in PC\u20103N cells and treatment of LNCaP cells with androgen and IGF\u20101.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MT1\u2010MMP expression was high in the apical regions of the luminal cells in PIN and prostate cancer and less intense in the basalateral regions of benign tissues. IGF\u20101R was expressed primarily in the basal cells of normal glands and highly expressed in prostate cancer. Inhibition of IGF\u20101R in PC\u20103N cells decreased MT1\u2010MMP expression and treatment of LNCaP cells with a synthetic androgen and IGF\u20101 increased MT1\u2010MMP expression.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>These data demonstrate that MT1\u2010MMP is highly expressed in the apical cytoplasmic regions of the luminal cells in PIN and prostate cancer when compared to basalateral cytoplasmic membrane staining in benign glands. Additionally, we demonstrate that IGF\u20101R is highly expressed in human prostate carcinoma. These findings suggest that MT1\u2010MMP localization and IGF\u20101R expression in prostate carcinoma could be predictive biomarkers for aggressive disease and support IGF\u20101R as a promising therapeutic target to decrease processes of prostate cancer metastasis. Prostate 68: 463\u2013476, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.20718","type":"journal-article","created":{"date-parts":[[2008,1,14]],"date-time":"2008-01-14T12:25:03Z","timestamp":1200313503000},"page":"463-476","source":"Crossref","is-referenced-by-count":19,"title":["Differential localization of MT1\u2010MMP in human prostate cancer tissue: Role of IGF\u20101R in MT1\u2010MMP expression"],"prefix":"10.1002","volume":"68","author":[{"given":"Isis C.","family":"Sroka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kathy","family":"McDaniel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond B.","family":"Nagle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G. 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