{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:39:44Z","timestamp":1790858384612,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2008,5,5]],"date-time":"2008-05-05T00:00:00Z","timestamp":1209945600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2008,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Folate and methyl\u2010group deficiency has been linked to prostate cancer susceptibility, yet the mechanisms underlying these observations are incompletely understood. The region of the genome containing the imprinted genes <jats:italic>insulin\u2010like growth factor 2<\/jats:italic> (<jats:italic>Igf2<\/jats:italic>) and <jats:italic>H19<\/jats:italic>, both of which display oncogenic functions, may be particularly sensitive to environmental influences.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To determine whether a methyl\u2010deficient diet impacts epigenetic controls at the <jats:italic>Igf2<\/jats:italic>\u2013<jats:italic>H19<\/jats:italic> locus, we placed C57BL\/6 mice containing a polymorphism at the imprinted <jats:italic>Igf2<\/jats:italic>\u2013<jats:italic>H19<\/jats:italic> locus on a choline and methionine deficient (CMD) diet. We interrogated this locus for expression and epigenetic changes in prostate tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A significant increase in both <jats:italic>Igf2<\/jats:italic> and <jats:italic>H19<\/jats:italic> expression was found in CMD prostate tissues compared to controls. These expression changes were reversible with shorter exposure to the CMD diet. Chromatin immunoprecipitation (ChIP) revealed significant decreases in repressive histone modifications (dimethyl\u2010H3K9) within the <jats:italic>H19<\/jats:italic> promoter, as well as <jats:italic>Igf2<\/jats:italic> P2 and P3 promoters. DNA methylation within these promoters was not altered. No significant change in <jats:italic>Igf2<\/jats:italic> or <jats:italic>H19<\/jats:italic> imprinting was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>These findings highlight the plasticity of the epigenome in an epithelial organ vulnerable to neoplastic change. They further suggest that chromatin modifications are more susceptible to methyl\u2010deficient diets than DNA methylation at this locus. Prostate 68: 1187\u20131195, 2008. \u00a9 2008 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.20782","type":"journal-article","created":{"date-parts":[[2008,5,5]],"date-time":"2008-05-05T14:20:51Z","timestamp":1209997251000},"page":"1187-1195","source":"Crossref","is-referenced-by-count":59,"title":["A methyl\u2010deficient diet modifies histone methylation and alters <i>Igf2<\/i> and <i>H19<\/i> repression in the prostate"],"prefix":"10.1002","volume":"68","author":[{"given":"Joseph R.","family":"Dobosy","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vivian X.","family":"Fu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua A.","family":"Desotelle","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rajini","family":"Srinivasan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michelle L.","family":"Kenowski","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nima","family":"Almassi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Richard","family":"Weindruch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John","family":"Svaren","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David F.","family":"Jarrard","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,5,5]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"crossref","first-page":"1060","DOI":"10.1093\/ajcn\/36.5.1060","article-title":"Folate nutrition in the elderly","volume":"36","author":"Rosenberg IH","year":"1982","journal-title":"Am J Clin Nutr"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/07315724.2003.10719270"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2003.155"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/aje\/kwj126"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2003.tb05974.x"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1997.445"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature05913"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2006.10.063"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/oxfordjournals.jbchem.a022661"},{"key":"e_1_2_1_11_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend.11.13.0030"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M405015200"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/17.6.1251"},{"key":"e_1_2_1_14_2","first-page":"4094","article-title":"Rapid appearance of hypomethylated DNA in livers of rats fed cancer\u2010promoting, methyl\u2010deficient diets","volume":"49","author":"Wainfan E","year":"1989","journal-title":"Cancer Res"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(02)00408-1"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003940070026"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0321006"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.15.5293-5300.2003"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2005"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2005-6-8-227"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/137.1.216S"},{"key":"e_1_2_1_22_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature02625"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.1996.tb00628.x"},{"key":"e_1_2_1_24_2","first-page":"3825","article-title":"Insulin\u2010like growth factor (IGF)\u2010I, IGF\u2010II and IGF type I receptor (IGFR\u2010I) expression in prostatic cancer","volume":"23","author":"Cardillo MR","year":"2003","journal-title":"Anticancer Res"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1096-6374(02)00121-1"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M504033200"},{"key":"e_1_2_1_27_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1471-4159.2006.04069.x"},{"key":"e_1_2_1_28_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-1832"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-03-3972"},{"key":"e_1_2_1_30_2","doi-asserted-by":"crossref","first-page":"2055","DOI":"10.1093\/genetics\/165.4.2055","article-title":"Age\u2010associated activation of epigenetically repressed genes in the mouse","volume":"165","author":"Bennett\u2010Baker PE","year":"2003","journal-title":"Genetics"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2042.1996.tb00628.x"},{"key":"e_1_2_1_32_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg022"},{"key":"e_1_2_1_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(00)00442-5"},{"key":"e_1_2_1_34_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2443.2006.01029.x"},{"key":"e_1_2_1_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrg2045"},{"key":"e_1_2_1_36_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.81.10.3774"},{"key":"e_1_2_1_37_2","doi-asserted-by":"publisher","DOI":"10.1054\/ghir.2000.0141"},{"key":"e_1_2_1_38_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1108080"},{"key":"e_1_2_1_39_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/23.11.1885"},{"key":"e_1_2_1_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgi364"},{"key":"e_1_2_1_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/5047"},{"key":"e_1_2_1_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.06.028"},{"key":"e_1_2_1_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.44.27863"},{"key":"e_1_2_1_44_2","doi-asserted-by":"publisher","DOI":"10.1210\/en.2003-0681"},{"key":"e_1_2_1_45_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.17.9733"},{"key":"e_1_2_1_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrfmmm.2005.06.028"},{"key":"e_1_2_1_47_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddi484"},{"key":"e_1_2_1_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1098-2744(199702)18:2<97::AID-MC5>3.0.CO;2-N"},{"key":"e_1_2_1_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/jn\/132.8.2333S"},{"key":"e_1_2_1_50_2","doi-asserted-by":"publisher","DOI":"10.1210\/jc.77.5.1203"},{"key":"e_1_2_1_51_2","doi-asserted-by":"crossref","first-page":"13779","DOI":"10.1016\/S0021-9258(17)36715-7","article-title":"Altered body composition and increased frequency of diverse malignancies in insulin\u2010like growth factor\u2010II transgenic mice","volume":"269","author":"Rogler CE","year":"1994","journal-title":"J Biol Chem"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.20782","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.20782","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,15]],"date-time":"2023-11-15T18:01:42Z","timestamp":1700071302000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.20782"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5,5]]},"references-count":50,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2008,8]]}},"alternative-id":["10.1002\/pros.20782"],"URL":"https:\/\/doi.org\/10.1002\/pros.20782","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5,5]]}}}