{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:28:06Z","timestamp":1762100886167},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,1,13]],"date-time":"2009-01-13T00:00:00Z","timestamp":1231804800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2009,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The CD133<jats:sup>hi<\/jats:sup> sub\u2010population of prostate epithelial cells has been demonstrated to possess tumor\u2010initiating capacity consistent with that of the cancer stem cell theory. However, the involvement of oncogenes such as c\u2010myc has not been fully elucidated in the CD133<jats:sup>hi<\/jats:sup> sub\u2010population.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We have isolated primary prostate cell strains (IBC\u201010a) and immortalized them by transfection with hTERT. The in vitro and in vivo tumorigenic capacity of isolated CD133<jats:sup>hi<\/jats:sup> and CD133<jats:sup>lo<\/jats:sup> cells was evaluated with respect to c\u2010myc expression using specific sense and anti\u2010sense oligonucleotides.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Freshly immortalized cells consisted of &lt;3.3% CD133<jats:sup>hi<\/jats:sup>\/CD24<jats:sup>hi<\/jats:sup> sub\u2010population (SP). \u201cProstaspheres\u201d generated from single CD133<jats:sup>hi<\/jats:sup> cells in the presence of EGF consisted of \u223c10% CD133<jats:sup>hi<\/jats:sup> SPs in 12\u201321 day cultures. A single Prostasphere generated from single CD133<jats:sup>hi<\/jats:sup> cells (6\u201310 cell stage at day 6 injected i.t) produced dysplastic lesions in NOD\u2010SCID mice (n\u2009=\u20094\/5). Treatment of Prostaspheres from CD133<jats:sup>hi<\/jats:sup> SPs in vitro with c\u2010myc or cyclin D1 anti\u2010sense oligonucleotides totally blocked colony forming ability and growth. Furthermore, treatment of fully formed, 6\u2010day Prostaspheres for 48 hr with c\u2010myc anti\u2010sense significantly reduced c\u2010myc expression and their ability to generate lesions in NOD\u2010SCIDs (n\u2009=\u200910 Prostaspheres injected i.t.\/mouse).<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These data demonstrate for the first time that a single CD133<jats:sup>hi<\/jats:sup> cell is competent to generate Prostaspheres in vitro and that CD133<jats:sup>hi<\/jats:sup> Prostaspheres require c\u2010myc to grow and form dysplastic lesions in vivo. Prostate 69: 689\u2013698, 2009. \u00a9 2009 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.20918","type":"journal-article","created":{"date-parts":[[2009,1,13]],"date-time":"2009-01-13T22:33:48Z","timestamp":1231886028000},"page":"689-698","source":"Crossref","is-referenced-by-count":12,"title":["Dysplasia of human prostate CD133<sup>hi<\/sup> sub\u2010population in NOD\u2010SCIDS is blocked by c\u2010myc 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