{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T16:51:14Z","timestamp":1791219074662,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,10,13]],"date-time":"2010-10-13T00:00:00Z","timestamp":1286928000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"European Community's Seventh Framework Programme","award":["FP7\/2007-2013"],"award-info":[{"award-number":["FP7\/2007-2013"]}]},{"name":"European Community's Seventh Framework Programme","award":["HEALTH-F2-2007-201438"],"award-info":[{"award-number":["HEALTH-F2-2007-201438"]}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100006383","name":"Cancer Society of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006383","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Reino Lahtikari Foundation"},{"name":"Sigrid Juselius Foundation"},{"name":"Medical Research Fund of Tampere University Hospital"},{"DOI":"10.13039\/501100003125","name":"Finnish Cultural Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003125","id-type":"DOI","asserted-by":"crossref"}]},{"name":"University of Tampere Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2011,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Androgens play a critical role in the growth of both androgen dependent and castration\u2010resistant prostate cancer (CRPC). Only a few micro\u2010RNAs (miRNAs) have been suggested to be androgen regulated. We aim to identify androgen regulated miRNAs.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We utilized LNCaP derived model, we have established, and which overexpresses the androgen receptor (AR), the VCaP cell line, and 13 intact\u2010castrated prostate cancer (PC) xenograft pairs, as well as clinical specimens of untreated (PC) and CRPC. The expression of miRNAs was analyzed by microarrays and quantitative RT\u2010PCR (Q\u2010RT\u2010PCR). Transfection of pre\u2010miR\u2010141 and anti\u2010miR\u2010141 was also used.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Seventeen miRNAs were &gt;1.5\u2010fold up\u2010 or downregulated upon dihydrotestosterone (DHT) treatment in the cell lines, and 42 after castration in the AR\u2010positive xenografts. Only four miRNAs (miR\u201010a, miR\u2010141, miR\u2010150*, and miR\u20101225\u20105p) showed similar androgen regulation in both cell lines and xenografts. Of those, miR\u2010141 was found to be expressed more in PC and CRPC compared to benign prostate hyperplasia. Additionally, the overexpression of miR\u2010141 enhanced growth of parental LNCaP cells while inhibition of miR\u2010141 by anti\u2010miR\u2010141 suppressed the growth of the LNCaP subline overexpressing AR.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>Only a few miRNAs were found to be androgen\u2010regulated in both cell lines and xenografts models. Of those, the expression of miR\u2010141 was upregulated in cancer. The ectopic overexpression of miR\u2010141 increased growth of LNCaP cell suggesting it may contribute to the progression of PC. Prostate 71:604\u2013614, 2011. \u00a9 2010 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.21276","type":"journal-article","created":{"date-parts":[[2010,10,14]],"date-time":"2010-10-14T13:37:04Z","timestamp":1287063424000},"page":"604-614","source":"Crossref","is-referenced-by-count":142,"title":["Androgen regulation of micro\u2010RNAs in prostate cancer"],"prefix":"10.1002","volume":"71","author":[{"given":"Kati K.","family":"Waltering","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kati P.","family":"Porkka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sanni E.","family":"Jalava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alfonso","family":"Urbanucci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pekka 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