{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:52:51Z","timestamp":1786978371808,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2011,2,14]],"date-time":"2011-02-14T00:00:00Z","timestamp":1297641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"crossref","award":["2P01 CA93900"],"award-info":[{"award-number":["2P01 CA93900"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100008788","name":"The Ohio State University College of Veterinary Medicine","doi-asserted-by":"crossref","award":["520026"],"award-info":[{"award-number":["520026"]}],"id":[{"id":"10.13039\/100008788","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2011,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>Prostate cancer in men has a high mortality and morbidity due to metastatic disease. The pathobiology of prostate cancer metastasis is not well understood and cell lines and animal models that recapitulate the complex nature of the disease are needed. Therefore, the goal of the study was to establish and characterize a new prostate cancer line derived from a dog with spontaneous prostate cancer.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>A new cell line (Leo) was derived from a dog with spontaneous prostate cancer. Immunohistochemistry and PCR were used to characterize the primary prostate cancer and xenografts in nude mice. Subcutaneous tumor growth and metastases in nude mice were evaluated by bioluminescent imaging, radiography and histopathology. In vitro chemosensitivity of Leo cells to therapeutic agents was measured.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>\n                      Leo cells expressed the secretory epithelial cytokeratins (CK)8, 18, and ductal cell marker, CK7. The cell line grew in vitro (over 75 passages) and was tumorigenic in the subcutis of nude mice. Following intracardiac injection, Leo cells metastasized to the brain, spinal cord, bone, and adrenal gland. The incidence of metastases was greatest to the central nervous system (80%) with a lower incidence to bone (20%) and the adrenal glands (16%). In vitro chemosensitivity assays demonstrated that Leo cells were sensitive to Velcade and an HDAC\u201042 inhibitor with IC\n                      <jats:sub>50<\/jats:sub>\n                      concentrations of 1.9\u2009nm and 0.95\u2009\u00b5m, respectively.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSION<\/jats:title>\n                    <jats:p>The new prostate cancer cell line (Leo) will be a valuable model to investigate the mechanisms of the brain and bone metastases. Prostate 71:1251\u20131263, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.21341","type":"journal-article","created":{"date-parts":[[2011,2,14]],"date-time":"2011-02-14T10:11:33Z","timestamp":1297678293000},"page":"1251-1263","source":"Crossref","is-referenced-by-count":39,"title":["Development of a brain metastatic canine prostate cancer cell line"],"prefix":"10.1002","volume":"71","author":[{"given":"Nanda K.","family":"Thudi","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sherry T.","family":"Shu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chelsea K.","family":"Martin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa G.","family":"Lanigan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murali V.P.","family":"Nadella","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrie","family":"Van Bokhoven","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jillian L.","family":"Werbeck","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessica K.","family":"Simmons","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sridhar","family":"Murahari","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William C.","family":"Kisseberth","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Breen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Williams","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ching\u2010Shih","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurie K.","family":"McCauley","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Evan T.","family":"Keller","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas J.","family":"Rosol","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,2,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/CA.2007.0010"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(19980615)36:1<64::AID-PROS12>3.0.CO;2-G"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0906-1065b"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20408"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1053\/hp.2000.6698"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022749"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-2442"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"4621","DOI":"10.1200\/jco.2006.24.18_suppl.4621","article-title":"Routine interval computed tomography in detecting new soft tissue disease in patients with androgen\u2010independent prostate cancer (AIPC) and only bone metastasis","volume":"24","author":"Wu S","year":"2006","journal-title":"J Clin Oncol"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.11522"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/s12254-008-0025-2"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20776"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910210305"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5829.2007.00139.x"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sc.2008.77"},{"key":"e_1_2_6_16_2","first-page":"2689","article-title":"Effects of calcitriol, seocalcitol, and medium\u2010chain triglyceride on a canine transitional cell carcinoma cell line","volume":"25","author":"Kaewsakhorn T","year":"2005","journal-title":"Anticancer Res"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1009224232134"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.189401"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1159\/000163088"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-5-65"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.2202\/1544-6115.1027"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1214\/07-AOAS102"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.1058"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1354\/vp.41-2-131"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1089\/dna.1991.10.49"},{"key":"e_1_2_6_27_2","first-page":"4028","article-title":"Expression of p53, Bcl\u20102, E\u2010cadherin, matrix metalloproteinase\u20109, and tissue inhibitor of metalloproteinases\u20101 in paired primary tumors and brain metastasis","volume":"5","author":"Arnold SM","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_28_2","first-page":"4959","article-title":"Expression of vascular endothelial growth factor is necessary but not sufficient for production and growth of brain metastasis 1","volume":"60","author":"Yano S","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0303-7207(02)00261-7"},{"key":"e_1_2_6_30_2","first-page":"237","article-title":"Pathobiology of brain metastases","volume":"58","author":"Nathoo N","year":"2005","journal-title":"Br Med J"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M210063200"},{"key":"e_1_2_6_32_2","doi-asserted-by":"crossref","first-page":"177","DOI":"10.1242\/jeb.132.1.177","article-title":"Nerve growth factor in neuronal development and maintenance","volume":"132","author":"Misko T","year":"1987","journal-title":"J Exp Biol"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ne.18.030195.001255"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20480"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi9626158"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygeno.2006.06.001"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1939-4640.1995.tb00534.x"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1210\/edrv-10-4-393"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0009-9120(03)00055-9"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20720"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbcan.2004.05.002"},{"key":"e_1_2_6_42_2","first-page":"7883","article-title":"Soluble receptor activator of nuclear factor \u03baB Fc diminishes prostate cancer progression in bone","volume":"63","author":"Zhang J","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.pcan.4500823"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.3324\/haematol.13285"},{"key":"e_1_2_6_45_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4626"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0429"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.21341","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.21341","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.21341","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T08:02:02Z","timestamp":1700553722000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.21341"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,14]]},"references-count":45,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2011,9]]}},"alternative-id":["10.1002\/pros.21341"],"URL":"https:\/\/doi.org\/10.1002\/pros.21341","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2,14]]}}}