{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:23:58Z","timestamp":1740104638287,"version":"3.37.3"},"reference-count":49,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2011,2,14]],"date-time":"2011-02-14T00:00:00Z","timestamp":1297641600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100009881","name":"Regione Liguria","doi-asserted-by":"crossref","award":["563\/2009"],"award-info":[{"award-number":["563\/2009"]}],"id":[{"id":"10.13039\/501100009881","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007388","name":"Compagnia San Paolo","doi-asserted-by":"crossref","award":["2009.1271"],"award-info":[{"award-number":["2009.1271"]}],"id":[{"id":"10.13039\/100007388","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ministero dell'Universita' e Ricerca Scientifica (MIUR) Project PRIN, Italy","award":["2005065404_003"],"award-info":[{"award-number":["2005065404_003"]}]},{"DOI":"10.13039\/501100003196","name":"Ministero della Salute","doi-asserted-by":"crossref","award":["RO\/8\/2007"],"award-info":[{"award-number":["RO\/8\/2007"]}],"id":[{"id":"10.13039\/501100003196","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2011,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Bicalutamide (BIC) is widely used in prostate cancer therapy. The dose and schedule employed are well tolerated, but about 50% of patients develop gynecomastia. Several studies have shown a significant reduction of the troublesome effects when Tamoxifen is concomitantly administered with BIC. However, the results reported in the literature seem to be preliminary and possible interferences could be present. In order to clarify the molecular mechanisms of the combination of the two drugs, we have investigated whether the expression of the proteins belonging to nuclear matrix (NM), one modulator of hormone action, is altered by BIC and\/or 4\u2010hydroxy\u2010tamoxifen (4OHT) in LNCaP cells. We focused above all on heterogeneous nuclear ribonucleoprotein K (hnRNP K) a NM protein with a key role in prostate carcinoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>NM proteins were analyzed by two\u2010dimensional gel electrophoresis. Modulation and compartmentalization of the androgen receptor and the hnRNP K were studied by Western blotting, confocal microscopy, and immunoprecipitation.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Proteomic analysis revealed that there is a similarity in the changes of the NM proteins elicited by drugs alone but that their combination does not result in a simple additive effect. Moreover, we found that in the nucleoplasm the androgen receptor and the hnRNP K colocalize in a complex that is highly proximal to DNA and that both proteins were synchronously modulated by BIC and\/or 4OHT treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSION<\/jats:title><jats:p>This study confirm the pivotal role of hnRNP K in prostate carcinoma and suggest that this role might be played by the interaction with the androgen receptor. Prostate 71:1466\u20131479, 2011. \u00a9 2011 Wiley\u2010Liss, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.21366","type":"journal-article","created":{"date-parts":[[2011,2,14]],"date-time":"2011-02-14T15:11:31Z","timestamp":1297696291000},"page":"1466-1479","source":"Crossref","is-referenced-by-count":8,"title":["Androgen receptor and heterogeneous nuclear ribonucleoprotein K colocalize in the nucleoplasm and are modulated by bicalutamide and 4\u2010hydroxy\u2010tamoxifen in prostatic cancer cell lines"],"prefix":"10.1002","volume":"71","author":[{"given":"Paola","family":"Barboro","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erica","family":"Repaci","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicoletta","family":"Ferrari","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alessandra","family":"Rubagotti","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Francesco","family":"Boccardo","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cecilia","family":"Balbi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,2,14]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2006.08.043"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(05)70464-2"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.12.013"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2009.05.019"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.01.032"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1430"},{"issue":"1","key":"e_1_2_6_8_2","first-page":"65","article-title":"Nuclear microenvironments: An architectural platform for the convergence and integration of transcriptional regulatory signals","volume":"48","author":"Stein GS","year":"2004","journal-title":"Eur J Histochem"},{"issue":"15","key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"7265","DOI":"10.1016\/S0021-9258(20)79697-3","article-title":"The specific binding of estrogens and androgens to the nuclear matrix of sex hormone responsive tissues","volume":"255","author":"Barrack ER","year":"1980","journal-title":"J Biol Chem"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.10218"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.10248"},{"issue":"6","key":"e_1_2_6_12_2","first-page":"3999","article-title":"Nuclear matrix protein expression in prostate cancer: Possible prognostic and diagnostic applications","volume":"25","author":"Barboro P","year":"2005","journal-title":"Anticancer Res"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20048"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-0155"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6603349"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207297"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-6017"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2308"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6605057"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.26.6.2187-2201.2006"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1006\/excr.2002.5605"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200305844"},{"issue":"1","key":"e_1_2_6_23_2","first-page":"13","article-title":"Differential proteomic analysis of nuclear matrix in muscle\u2010invasive bladder cancer: Potential to improve diagnosis and prognosis","volume":"30","author":"Barboro P","year":"2008","journal-title":"Cell Oncol"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/elps.1150090106"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/227680a0"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-4644(20001201)79:3<471::AID-JCB120>3.0.CO;2-O"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2008.10.017"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.0346-04.2004"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.1993.tb03313.x"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2818.2006.01706.x"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1117\/1.1631315"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1042\/BST0341124"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0388"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21074"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1210\/mend.16.4.0812"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20593"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.21834"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M203310200"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M805980200"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.24305"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.5.3619"},{"issue":"8","key":"e_1_2_6_42_2","doi-asserted-by":"crossref","first-page":"1162","DOI":"10.1210\/mend.14.8.0497","article-title":"Dynamics of intracellular movement and nucleocytoplasmic recycling of the ligand\u2010activated androgen receptor in living cells","volume":"14","author":"Tyagi RK","year":"2000","journal-title":"Mol Endocrinol"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-2952(01)00927-3"},{"issue":"6","key":"e_1_2_6_44_2","first-page":"4277","article-title":"4\u2010OH tamoxifen does not interfere with bicalutamide inhibitory effects on human prostatic cancer cells in vitro","volume":"25","author":"Boccardo F","year":"2005","journal-title":"Anticancer Res"},{"issue":"6","key":"e_1_2_6_45_2","first-page":"2462","article-title":"Inhibition of protein kinase C by Tamoxifen","volume":"45","author":"O'Brian C","year":"1985","journal-title":"Cancer Res"},{"key":"e_1_2_6_46_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.21.15101"},{"key":"e_1_2_6_47_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.pcan.4500782"},{"key":"e_1_2_6_48_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.01.031"},{"key":"e_1_2_6_49_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00280-005-1016-1"},{"issue":"12","key":"e_1_2_6_50_2","first-page":"3175","article-title":"Expression of estrogen receptor (ER)\u2010\u03b1 and ER\u2010\u03b2 in normal and malignant prostatic epithelial cells: Regulation by methylation and involvement in growth regulation","volume":"60","author":"Lau KM","year":"2000","journal-title":"Cancer Res"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.21366","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.21366","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.21366","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T12:35:12Z","timestamp":1700570112000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.21366"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2,14]]},"references-count":49,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2011,9,15]]}},"alternative-id":["10.1002\/pros.21366"],"URL":"https:\/\/doi.org\/10.1002\/pros.21366","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"type":"print","value":"0270-4137"},{"type":"electronic","value":"1097-0045"}],"subject":[],"published":{"date-parts":[[2011,2,14]]}}}