{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:21:42Z","timestamp":1788870102917,"version":"build-2803163510"},"reference-count":29,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2011,5,31]],"date-time":"2011-05-31T00:00:00Z","timestamp":1306800000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Program for Changjiang Scholars and Innovative Research Team in University","award":["0849"],"award-info":[{"award-number":["0849"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA121277"],"award-info":[{"award-number":["CA121277"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA125747"],"award-info":[{"award-number":["CA125747"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA091956"],"award-info":[{"award-number":["CA091956"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA130908"],"award-info":[{"award-number":["CA130908"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["CA134514"],"award-info":[{"award-number":["CA134514"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000005","name":"Department of Defense","doi-asserted-by":"crossref","award":["W81XWH-09-1-622"],"award-info":[{"award-number":["W81XWH-09-1-622"]}],"id":[{"id":"10.13039\/100000005","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2012,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>BACKGROUND<\/jats:title>\n                    <jats:p>\n                      Androgens control homeostasis of the normal prostate and growth of prostate cancer (PCa) through the androgen receptor (AR) by regulating gene networks involving in cell proliferation, differentiation, and survival. We demonstrated previously that expression of Skp2, a key protein regulating cell entry into the S phase, is inhibited by androgens in an AR\u2010dependent manner (\n                      <jats:italic>Oncogene<\/jats:italic>\n                      , 2004; 23(12): 2161\u20132176). However, the underlying mechanism of this regulation is unknown.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>METHODS<\/jats:title>\n                    <jats:p>Using the LNCaP PCa cell line as a working model, the effect of androgens on the expression of Skp2 was examined by Western and Northern blot analyses. Cell cycle was measured by fluorescence\u2010activated cell sorting (FACS). Gene transfection was performed by electroporation to manipulate the expression levels of proteins studied.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>RESULTS<\/jats:title>\n                    <jats:p>At physiological levels androgens markedly repressed Skp2 expression but slightly induced Skp2 expression at subphysiological levels. Androgens modestly decreased the stability of the Skp2 protein. Androgenic repression of Skp2 expression was completely abolished by E1A\u2010mediated inactivation of pocket proteins including RB, p130, and p107. Moreover, ectopic expression of p107 inhibited Skp2 expression, and silencing of p107 partially blocked androgenic repression of Skp2.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>CONCLUSIONS<\/jats:title>\n                    <jats:p>Our data indicate that androgens repress Skp2 expression via p107\u2010dependent and \u2010independent pathways in PCa cells. These regulatory mechanisms may be targeted for the development of new therapeutics of androgen\u2010refractory PCa. Prostate 72:225\u2013232, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.21430","type":"journal-article","created":{"date-parts":[[2011,5,31]],"date-time":"2011-05-31T08:57:06Z","timestamp":1306832226000},"page":"225-232","source":"Crossref","is-referenced-by-count":20,"title":["Androgens repress expression of the f\u2010box protein Skp2 via p107 dependent and independent mechanisms in LNCaP prostate cancer cells"],"prefix":"10.1002","volume":"72","author":[{"given":"Jingting","family":"Jiang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunqian","family":"Pan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin M.","family":"Regan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changping","family":"Wu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xueguang","family":"Zhang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donald J.","family":"Tindall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haojie","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,5,31]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990050510"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-122-2-552"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207326"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.03.4777"},{"key":"e_1_2_6_6_2","first-page":"6940","article-title":"Expression of the protooncogene bcl\u20102 in the prostate and its association with emergence of androgen\u2010independent prostate cancer","volume":"52","author":"McDonnell TJ","year":"1992","journal-title":"Cancer 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Skp2 protein expression in human prostate cancer: Association with loss of the cyclin\u2010dependent kinase inhibitor p27 and PTEN and with reduced recurrence\u2010free survival","volume":"8","author":"Yang G","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000072113.34524.a7"},{"key":"e_1_2_6_20_2","first-page":"1583","article-title":"Expression of the F\u2010box protein SKP2 induces hyperplasia, dysplasia, and low\u2010grade carcinoma in the mouse prostate","volume":"63","author":"Shim EH","year":"2003","journal-title":"Cancer 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