{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:08:04Z","timestamp":1790860084572,"version":"4.1.0"},"reference-count":23,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2011,8,31]],"date-time":"2011-08-31T00:00:00Z","timestamp":1314748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2012,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Rodent models are often suboptimal for translational research on human prostate cancer (PCa). To better fill the gap with human, we refined the previously described orthotopic dog prostate cancer (DPC)\u20101 model.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Cyclosporine (Cy) A was used for immune suppression at varying doses and time\u2010periods prior and after orthotopic DPC\u20101 cell implantation in the dog prostate (n\u2009=\u200912). Follow up included digital rectal examination, ultrasound prostate imaging and biopsies of hypoechoic areas. At necropsy, the prostate, iliosacral lymph nodes (LN), lung nodules, and suspicious bone segments were collected for histopathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>15\u2009mg\u2009CyA\/kg daily for 10 days was optimal for tumor take. Maintaining these conditions post\u2010implantation resulted in a rapid tumor development within and beyond the prostate and in iliosacral LNs. To minimize tumor burden, 10 times less DPC\u20101 cells were implanted. A series of dogs was next followed for 3\u20134 months, under continuous immune suppression (n\u2009=\u20093) or with CyA interruption at 8.5 weeks (n\u2009=\u20092). In all instances, multifocal tumors were found within the prostate. Predominant patterns were micropapillary and cribriform. Metastases were present in iliosacral LNs and lungs. Moreover, pelvic bone metastases producing a mixed osteoblastic\/osteolytic reaction were confirmed in two dogs, one per group. Lastly, the release of CyA 1\u20132 weeks post\u2010implantation (n\u2009=\u20093) did not prevent tumor growth and spreading to LNs.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>The continuing growth of DPC\u20101 tumors despite the release of CyA and, for the first time, spreading to bones renders this refined model closer to the spontaneous canine and hormone\u2010refractory phase of human PCa. Prostate 72:752\u2013761, 2012. \u00a9 2011 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.21479","type":"journal-article","created":{"date-parts":[[2011,9,1]],"date-time":"2011-09-01T07:12:04Z","timestamp":1314861124000},"page":"752-761","source":"Crossref","is-referenced-by-count":17,"title":["Refining the orthotopic dog prostate cancer (DPC)\u20101 model to better bridge the gap between rodents and men"],"prefix":"10.1002","volume":"72","author":[{"given":"Maurice","family":"Anidjar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Scarlata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabio L.","family":"Cury","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joice","family":"Rocha","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucie","family":"Hamel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Murilo","family":"Luz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone","family":"Chevalier","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,8,31]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20121"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.5489\/cuaj.10160"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000169133.82167.aa"},{"key":"e_1_2_7_5_2","first-page":"152","article-title":"Benign hypertrophy and carcinoma of the prostate","volume":"16","author":"Moore RA","year":"1944","journal-title":"Surgery"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-0045(19980615)36:1<64::AID-PROS12>3.0.CO;2-G"},{"key":"e_1_2_7_7_2","first-page":"1687","volume-title":"Textbook of Veterinary Internal Medicine. 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