{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T17:25:51Z","timestamp":1791307551296,"version":"4.3.1"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,11,26]],"date-time":"2012-11-26T00:00:00Z","timestamp":1353888000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Inflammation is commonly observed in radical prostatectomy specimens, and evidence suggests that inflammation may contribute to prostate carcinogenesis. Multiple microorganisms have been implicated in serving as a stimulus for prostatic inflammation. The pro\u2010inflammatory anaerobe, <jats:italic>Propionibacterium acnes<\/jats:italic>, is ubiquitously found on human skin and is associated with the skin disease acne vulgaris. Recent studies have shown that <jats:italic>P. acnes<\/jats:italic> can be detected in prostatectomy specimens by bacterial culture or by culture\u2010independent molecular techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Radical prostatectomy tissue samples were obtained from 30 prostate cancer patients and subject to both aerobic and anaerobic culture. Cultured species were identified by 16S rDNA gene sequencing. <jats:italic>Propionibacterium acnes<\/jats:italic> isolates were typed using multilocus sequence typing (MLST).<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Our study confirmed that <jats:italic>P. acnes<\/jats:italic> can be readily cultured from prostatectomy tissues (7 of 30 cases, 23%). In some cases, multiple isolates of <jats:italic>P. acnes<\/jats:italic> were cultured as well as other <jats:italic>Propionibacterium<\/jats:italic> species, such as <jats:italic>P. granulosum<\/jats:italic> and <jats:italic>P. avidum<\/jats:italic>. Overall, 9 of 30 cases (30%) were positive for <jats:italic>Propionibacterium<\/jats:italic> spp. MLST analyses identified eight different sequence types (STs) among prostate\u2010derived <jats:italic>P. acnes<\/jats:italic> isolates. These STs belong to two clonal complexes, namely CC36 (type I\u20102) and CC53\/60 (type II), or are CC53\/60\u2010related singletons.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>MLST typing results indicated that prostate\u2010derived <jats:italic>P. acnes<\/jats:italic> isolates do not fall within the typical skin\/acne STs, but rather are characteristic of STs associated with opportunistic infections and\/or urethral flora. The MLST typing results argue against the likelihood that prostatectomy\u2010derived <jats:italic>P. acnes<\/jats:italic> isolates represent contamination from skin flora. Prostate 73: 770\u2013777, 2013. \u00a9 2012 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.22621","type":"journal-article","created":{"date-parts":[[2012,11,26]],"date-time":"2012-11-26T15:43:03Z","timestamp":1353944583000},"page":"770-777","source":"Crossref","is-referenced-by-count":60,"title":["Multilocus sequence typing (MLST) analysis of <i>Propionibacterium acnes<\/i> isolates from radical prostatectomy specimens"],"prefix":"10.1002","volume":"73","author":[{"given":"Tim N.","family":"Mak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shu\u2010Han","family":"Yu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Angelo M.","family":"De 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