{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T05:47:57Z","timestamp":1781848077058,"version":"3.54.5"},"reference-count":29,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2014,8,23]],"date-time":"2014-08-23T00:00:00Z","timestamp":1408752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>The U.S. FDA has approved several novel systemic agents including abiraterone acetate and taxoid cabazitaxel for metastatic castration\u2010resistant prostate cancer (CRPC) result in a complicated decision\u2010making while selecting an appropriate treatment. Therefore, a predictive biomarker for CRPC would provide useful information to physicians. The aim of this study is to evaluate the diagnostic potential of serum <jats:italic>N<\/jats:italic>\u2010glycan profiling in CRPC.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Serum <jats:italic>N<\/jats:italic>\u2010glycomics was performed in 80 healthy volunteers and 286 benign prostatic hyperplasia, 258 early\u2010stage PC, 46 PC with androgen deprivation therapy (ADT), and 68 CRPC patients using the glycoblotting method. A total of 36 types of <jats:italic>N<\/jats:italic>\u2010glycan levels in each patient were analyzed using logistic regression analysis and receiver operating characteristic curves. We also examined the expression of <jats:italic>N<\/jats:italic>\u2010glycan branching enzyme genes in PC cell lines using quantitative RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>We observed that tri\u2010 and tetra\u2010antennary <jats:italic>N<\/jats:italic>\u2010glycans were significantly higher in CRPC patients than in any other groups. The longitudinal follow\u2010up of tri\u2010 and tetra\u2010 antennary <jats:italic>N<\/jats:italic>\u2010glycan levels revealed that one PC with ADT patient showed an increase that was more than the cut\u2010off level and two consecutive increases in tri\u2010 and tetra\u2010antennary <jats:italic>N<\/jats:italic>\u2010glycan levels 3 months apart; resulted in biochemical recurrence despite the castrate level of testosterone, and the patient was defined as CRPC. Expression of <jats:italic>N<\/jats:italic>\u2010glycan branching enzyme genes were significantly upregulated in CRPC cell lines.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>These results suggest that the overexpression of tri\u2010 and tetra\u2010antennary <jats:italic>N<\/jats:italic>\u2010glycan may be associated with the castration\u2010resistant status in PC and may be a potential predictive biomarker for CRPC. <jats:italic>Prostate 74:1521\u20131529, 2014<\/jats:italic>. \u00a9 2014 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.22869","type":"journal-article","created":{"date-parts":[[2014,8,24]],"date-time":"2014-08-24T00:22:58Z","timestamp":1408839778000},"page":"1521-1529","source":"Crossref","is-referenced-by-count":52,"title":["Serum tri\u2010 and tetra\u2010antennary <i>N<\/i>\u2010glycan is a potential predictive biomarker for castration\u2010resistant prostate cancer"],"prefix":"10.1002","volume":"74","author":[{"given":"Yusuke","family":"Ishibashi","sequence":"first","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuki","family":"Tobisawa","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Hatakeyama","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsu","family":"Ohashi","sequence":"additional","affiliation":[{"name":"Graduate School of Life Science and Frontier Research Center for Post\u2010Genome Science and Technology Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Medicinal Chemistry Pharmaceuticals, Co. Ltd, Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shintaro","family":"Narita","sequence":"additional","affiliation":[{"name":"Department of Urology Akita University Graduate School of Medicine Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takuya","family":"Koie","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonori","family":"Habuchi","sequence":"additional","affiliation":[{"name":"Department of Urology Akita University Graduate School of Medicine Akita Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shin\u2010Ichiro","family":"Nishimura","sequence":"additional","affiliation":[{"name":"Graduate School of Life Science and Frontier Research Center for Post\u2010Genome Science and Technology Hokkaido University Sapporo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chikara","family":"Ohyama","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"},{"name":"Department of Advanced Transplant and Regenerative Medicine Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tohru","family":"Yoneyama","sequence":"additional","affiliation":[{"name":"Department of Urology Hirosaki University Graduate School of Medicine Hirosaki Japan"},{"name":"Department of Advanced Transplant and Regenerative Medicine Hirosaki University Graduate School of Medicine Hirosaki Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,8,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20138"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canep.2012.07.012"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-1241.2011.02799.x"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/aja.2013.38"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1014618"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa1001294"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)61389-X"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(14)70129-9"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-1938"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25117"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwi086"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20883"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm094"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21728"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910490303"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2013.10.052"},{"key":"e_1_2_7_19_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2013\/268407","article-title":"Serum N\u2010glycan profiling predicts prognosis in patients undergoing hemodialysis","volume":"5","author":"Hatakeyama S","year":"2013","journal-title":"Sci World J"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26531"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac101599p"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1074\/mcp.M900559-MCP200"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/ac702124d"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26262"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-012-0732-7"},{"key":"e_1_2_7_26_1","first-page":"1","article-title":"N\u2010 and O\u2010glycome analysis of serum and urine from bladder cancer patients using a high\u2010throughput glycoblotting method","volume":"3","author":"Takeuchi M","year":"2013","journal-title":"J Glycomics Lipidomics"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1651-2227.2006.00178.x"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/pr060664t"},{"key":"e_1_2_7_29_1","first-page":"1993","article-title":"Evaluating the function of matriptase and N\u2010acetylglucosaminyltransferase V in prostate cancer metastasis","volume":"28","author":"Tsui KH","year":"2008","journal-title":"Anticancer Res"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0043721"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.22869","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.22869","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T13:00:47Z","timestamp":1696510847000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.22869"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,8,23]]},"references-count":29,"journal-issue":{"issue":"15","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1002\/pros.22869"],"URL":"https:\/\/doi.org\/10.1002\/pros.22869","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,8,23]]}}}