{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T03:39:01Z","timestamp":1789702741592,"version":"4.0.1"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,1,18]],"date-time":"2015-01-18T00:00:00Z","timestamp":1421539200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002338","name":"Ministry of Education of China","doi-asserted-by":"crossref","award":["NO.IRT1111"],"award-info":[{"award-number":["NO.IRT1111"]}],"id":[{"id":"10.13039\/501100002338","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The National Basic Research Program of China","award":["2012CB518300"],"award-info":[{"award-number":["2012CB518300"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81430058"],"award-info":[{"award-number":["81430058"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81101946"],"award-info":[{"award-number":["81101946"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81472397"],"award-info":[{"award-number":["81472397"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81172447"],"award-info":[{"award-number":["81172447"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"The Shanghai Pujiang Program","award":["12PJD008"],"award-info":[{"award-number":["12PJD008"]}]},{"name":"Prostate Cancer Foundation Young Investigator Award"},{"name":"Shanghai Municipal Health and Family Planning Commission Outstanding Young Investigator","award":["XYQ2013077"],"award-info":[{"award-number":["XYQ2013077"]}]},{"DOI":"10.13039\/501100003395","name":"Shanghai Municipal Education Commission","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003395","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007219","name":"Shanghai Natural Science Foundation","doi-asserted-by":"crossref","award":["11ZR1447800"],"award-info":[{"award-number":["11ZR1447800"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Long non\u2010coding RNA (LncRNA) PCA3 has been a well\u2010established urine biomarker for the detection of prostate cancer (PCa). Our previous study showed a novel LncRNA FR0348383 is up\u2010regulated in over 70% of PCa compared with matched benign tissues. The aim of this study was to evaluate the diagnostic value of urinary FR0348383 for men undergoing prostate biopsy due to elevated PSA (PSA\u2009&gt;\u20094.0\u2009ng\/ml) and\/or abnormal digital rectal examination (DRE).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Post\u2010DRE first\u2010catch urine specimens prior to prostate biopsies were prospectively collected. After the whole transcriptome amplification, quantitative real time polymerase chain reaction was applied to quantify urine FR0348383 and PSA levels. The FR0348383 score was calculated as the ratio of PSA and FR0348383 mRNA (PSA mRNA\/FR0348383 mRNA\u2009\u00d7\u20091000). The diagnostic value of FR0348383 score was evaluated by logistic regression and decision curve analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>213 cases with urine samples containing sufficient mRNA were included, 94 cases had serum PSA level 4.0\u201310.0\u2009ng\/ml. PCa was identified in 72 cases. An increasing FR0348383 score was correlated with an increasing probability of a positive biopsy (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Multivariable logistic analysis indicated FR0348383 score (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), PSA (<jats:italic>P<\/jats:italic>\u2009=\u20090.004), age (<jats:italic>P<\/jats:italic>\u2009=\u20090.007), prostate volume (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) were independent predictors of PCa. ROC analysis demonstrated FR0348383 score outperformed PSA, %free PSA, and PSA Density in the prediction of PCa in the subgroup of patients with grey area PSA (AUC: 0.815 vs. 0.562 vs. 0.599 vs. 0.645). When using a probability threshold of 30% in the grey zone cohort, The FR0348383 score would save 52.0% of avoidable biopsies without missing any high grade cancers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>FR0348383 transcript in post\u2010DRE urine may be a novel biomarker for detection of PCa with great diagnostic value, especially in the grey zone cohort. The application of FR0348383 score in clinical practice might avoid unnecessary prostate biopsies and increase the specificity of PCa diagnosis. <jats:italic>Prostate 75: 653\u2013661, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.22949","type":"journal-article","created":{"date-parts":[[2015,1,18]],"date-time":"2015-01-18T19:41:14Z","timestamp":1421610074000},"page":"653-661","source":"Crossref","is-referenced-by-count":42,"title":["A novel urinary long non\u2010coding RNA transcript improves diagnostic accuracy in patients undergoing prostate biopsy"],"prefix":"10.1002","volume":"75","author":[{"given":"Wei","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shan\u2010Cheng","family":"Ren","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Lei","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Health Division of Guard Bureau General Staff Department of Chinese People's Liberation Army Beijing P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya\u2010Sheng","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tai\u2010Le","family":"Jing","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fu\u2010Bo","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rui","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chuan\u2010Liang","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui\u2010Qing","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hai\u2010Feng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yao\u2010Ming","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zi\u2010Yu","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fei","family":"Guo","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Shen","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xu","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jian\u2010Guo","family":"Hou","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying\u2010Hao","family":"Sun","sequence":"additional","affiliation":[{"name":"Department of Urology Changhai Hospital The Second Military Medical University Shanghai P.R.China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,1,18]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2013.09.046"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2013.02.3184"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200105033441806"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa0810084"},{"key":"e_1_2_7_7_1","first-page":"5975","article-title":"D D3: A new prostate\u2013specific gene, highly overexpressed in prostate cancer","volume":"59","author":"Bussemakers MJ","year":"1999","journal-title":"Cancer Res"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2013.04.026"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/cr.2012.30"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1177\/0272989X06295361"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2013.12.005"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22563"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3109\/0284186X.2010.524935"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2005.063289"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2010.06.039"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21390"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.06625"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrurol.2009.40"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djk110"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-3283"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2012.11.014"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn326"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2008.12.018"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa031918"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.4.523"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.22949","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.22949","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.22949","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T07:05:10Z","timestamp":1696403110000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.22949"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2015,1,18]]},"references-count":24,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2015,5]]}},"alternative-id":["10.1002\/pros.22949"],"URL":"https:\/\/doi.org\/10.1002\/pros.22949","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2015,1,18]]}}}