{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:24:15Z","timestamp":1740104655773,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2015,2,8]],"date-time":"2015-02-08T00:00:00Z","timestamp":1423353600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["24592388"],"award-info":[{"award-number":["24592388"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2015,6]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>Recent studies have reported that bone marrow\u2010derived cells (BMDCs), which are recruited to sites of tissue injury and inflammation, can differentiate into epithelial cells, such as liver, lung, gastrointestinal tract, and skin cells. We investigated the role of BMDCs in contributing to regeneration of injured prostate epithelium.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>Using chimera rats that received allogenic bone marrow grafts from green fluorescent protein (GFP) transgenic rats after lethal whole\u2010body irradiation, we investigated the existence of epithelial marker\u2010positive BMDCs in injured prostate tissue caused by transurethral injection of lipopolysaccharide.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>Prostate tissues were harvested 2 weeks after transurethral lipopolysaccharide injection. Immunofluorescence staining showed that some cells in the stroma co\u2010expressed GFP and pan\u2010cytokeratin, which suggested the existence of epithelial marker\u2010positive BMDCs. To confirm the existence of such cells, we collected bone marrow\u2010derived non\u2010hematopoietic cells (GFP+\/CD45\u2212 cells) from the prostate by fluorescence\u2010activated cell sorter analysis and analyzed the characteristics of the GFP+\/CD45\u2212 cells. The number of cells in this population significantly increased from 0.042% to 0.492% compared with normal prostate tissue. We found by immunofluorescent analysis and RT\u2010PCR that GFP+\/CD45\u2212 cells expressed cytokeratin, which suggested that these cells have some features of epithelial cells. In the prostate obtained from the chimera rats 34 weeks after lipopolysaccharide injection, GFP\u2010 and cytokeratin\u2010positive cells were observed in the prostate gland, which suggested that some of the cells in the prostate gland regenerated after prostate inflammation derived from bone marrow.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>BMDCs might be able to differentiate into prostate epithelial cells after prostatic injury. <jats:italic>Prostate 75:806\u2013814, 2015<\/jats:italic>. \u00a9 2015 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.22962","type":"journal-article","created":{"date-parts":[[2015,2,8]],"date-time":"2015-02-08T16:27:20Z","timestamp":1423412840000},"page":"806-814","source":"Crossref","is-referenced-by-count":3,"title":["Bone marrow\u2010derived cells contribute to regeneration of injured prostate epithelium and stroma"],"prefix":"10.1002","volume":"75","author":[{"given":"Wataru","family":"Nakata","sequence":"first","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasutomo","family":"Nakai","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka Medical Center for Cancer and Cardiovascular Diseases Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takahiro","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mototaka","family":"Sato","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Koji","family":"Hatano","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Nagahara","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazutoshi","family":"Fujita","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Motohide","family":"Uemura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norio","family":"Nonomura","sequence":"additional","affiliation":[{"name":"Department of Urology Osaka University Graduate School of Medicine Osaka 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