{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:27:10Z","timestamp":1778657230330,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T00:00:00Z","timestamp":1487808000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81472382"],"award-info":[{"award-number":["81472382"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81101947"],"award-info":[{"award-number":["81101947"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"National Cancer Institute","doi-asserted-by":"publisher","award":["R01CA142862"],"award-info":[{"award-number":["R01CA142862"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>BACKGROUND<\/jats:title><jats:p>SHARPIN, SHANK\u2010associated RH domain interacting protein, associates with a linear ubiquitin chain assembly complex (LUBAC) to regulate inflammation and immunity. It has been reported that SHARPIN is highly expressed in several human tumors including ovarian cancer and liver cancer. We found that SHARPIN is also highly expressed in prostate cancer cell lines of DU145, LNCAP, and PC\u20103. Suppression of SHARPIN caused an inhibition of NF\u2010\u03baB signal and decreases in tumorigenesis of cultured cells in NOD\/SCID mouse model. Overexpression of SHARPIN in prostate cancer cells promoted cell growth and reduced apoptosis through NF\u2010kB\/ERK\/Akt pathway and apoptosis\u2010associated proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>METHODS<\/jats:title><jats:p>We analyzed the expression of SHARPIN in prostate cancer tissues from 95 patients and its relationship with other clinical characteristics associated with PCA malignancies and patient survivals, and examined the impacts of SHARPIN suppression with siRNA on proliferation, angiogenesis, invasion, and expression levels of MMP\u20109 of prostate cancer cells and metastasis to lung by these cells in nude mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>RESULTS<\/jats:title><jats:p>High levels of SHARPIN were associated with high malignancies of PCA and predicted shorter survivals of PCA patients. Suppression of SHARPIN impaired cell proliferation, angiogenesis, and invasion and reduced levels of MMP\u20109 in prostate cancer cells and reduced the size of metastatic lung tumors induced by these cells in mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>CONCLUSIONS<\/jats:title><jats:p>SHARPIN enhances the metastasis of prostate cancer and impair patient survivals. <jats:italic>Prostate 77:718\u2013728, 2017<\/jats:italic>. \u00a9 2017 Wiley Periodicals, Inc.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.23302","type":"journal-article","created":{"date-parts":[[2017,2,23]],"date-time":"2017-02-23T10:18:41Z","timestamp":1487845121000},"page":"718-728","source":"Crossref","is-referenced-by-count":13,"title":["Elevation of SHARPIN Protein Levels in Prostate Adenocarcinomas Promotes Metastasis and Impairs Patient Survivals"],"prefix":"10.1002","volume":"77","author":[{"given":"Hai","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Urology, The Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"},{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"},{"name":"Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology Texas A&amp;M University Houston Texas"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tao","family":"Du","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation, Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"},{"name":"Center for Cancer and Stem Cell Biology, Institute of Biosciences and Technology Texas A&amp;M University Houston Texas"},{"name":"Department of Gynecology &amp; Obstetrics, Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou 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