{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,8]],"date-time":"2025-11-08T13:04:14Z","timestamp":1762607054196,"version":"3.37.3"},"reference-count":16,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T00:00:00Z","timestamp":1500508800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["1R01NS076991\u201001, P01HL59407\u201011, P01AI100263\u201001"],"award-info":[{"award-number":["1R01NS076991\u201001, P01HL59407\u201011, P01AI100263\u201001"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004572","name":"National Health and Family Planning Commission of the People's Republic of China","doi-asserted-by":"publisher","award":["17PJ583"],"award-info":[{"award-number":["17PJ583"]}],"id":[{"id":"10.13039\/501100004572","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004912","name":"Sichuan University","doi-asserted-by":"publisher","award":["2012ZX10004\u2010901"],"award-info":[{"award-number":["2012ZX10004\u2010901"]}],"id":[{"id":"10.13039\/501100004912","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Prostate diseases are common in males worldwide with high morbidity. Gene therapy is an attractive therapeutic strategy for prostate diseases, however, it is currently underdeveloped. As well known, adeno virus (Ad) is the most widely used gene therapy vector. The aims of this study are to explore transduction efficiency of Ad in prostate cancer cells and normal prostate tissue, thus further providing guidance for future prostate pathophysiological studies and therapeutic development of prostate diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We produced Ad expressing enhanced green fluorescence protein (EGFP), and characterized the transduction efficiency of Ad in both human and mouse prostate cancer cell lines in vitro, as well as prostate tumor xenograft, and wild\u2010type mouse prostate tissue in vivo. Ad transduction efficiency was determined by EGFP fluorescence using microscopy and flow cytometry. Cell type\u2010specific transduction was examined by immunofluorescence staining of cell markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data showed that Ad efficiently transduced human and mouse prostate cancer cells in vitro in a dose dependent manner. Following intratumoral and intraprostate injection, Ad could efficiently transduce prostate tumor xenograft and the major prostatic cell types in vivo, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our findings suggest that Ad can efficiently transduce prostate tumor cells in vitro as well as xenograft and normal prostate tissue in vivo, and further indicate that Ad could be a potentially powerful toolbox for future gene therapy of prostate diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.23386","type":"journal-article","created":{"date-parts":[[2017,7,20]],"date-time":"2017-07-20T05:50:43Z","timestamp":1500529843000},"page":"1265-1270","source":"Crossref","is-referenced-by-count":2,"title":["Characterization of adenoviral transduction profile in prostate cancer cells and normal prostate tissue"],"prefix":"10.1002","volume":"77","author":[{"given":"Jianzhong","family":"Ai","sequence":"first","affiliation":[{"name":"Institute of Urology, Department of Urology West China Hospital Sichuan University Chengdu Sichuan P.R. China"},{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"},{"name":"Department of Microbiology and Physiology Systems University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Phillip W.L.","family":"Tai","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi","family":"Lu","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"},{"name":"Shanghai General Hospital Shanghai Jiaotong University Shanghai P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Li","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Ma","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qin","family":"Su","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qiang","family":"Wei","sequence":"additional","affiliation":[{"name":"Institute of Urology, Department of Urology West China Hospital Sichuan University Chengdu Sichuan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Urology, Department of Urology West China Hospital Sichuan University Chengdu Sichuan P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0097-9012","authenticated-orcid":false,"given":"Guangping","family":"Gao","sequence":"additional","affiliation":[{"name":"Horae Gene Therapy Center University of Massachusetts Medical School Worcester Massachusetts"},{"name":"Department of Microbiology and Physiology Systems University of Massachusetts Medical School Worcester Massachusetts"},{"name":"State Key Laboratory of Biotherapy West China Hospital, Sichuan University Chengdu P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,20]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ecl.2011.05.012"},{"key":"e_1_2_8_3_1","first-page":"S90","article-title":"The cost of treating the 10 most prevalent diseases in men 50 years of age or older","volume":"12","author":"Fenter TC","year":"2006","journal-title":"Am J Manag Care"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2016.05.043"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.12310"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(13)70184-0"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2011.08.029"},{"key":"e_1_2_8_9_1","first-page":"5031","article-title":"The dual impact of coxsackie and adenovirus receptor expression on human prostate cancer gene therapy","volume":"60","author":"Okegawa T","year":"2000","journal-title":"Cancer Res"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-84-20714"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/jgm.2698"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.76.9.4612-4620.2002"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2014.110"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2013.40"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1189992"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08361"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.rcl.2006.07.005"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.23386","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.23386","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T12:50:09Z","timestamp":1696510209000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.23386"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,20]]},"references-count":16,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1002\/pros.23386"],"URL":"https:\/\/doi.org\/10.1002\/pros.23386","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"type":"print","value":"0270-4137"},{"type":"electronic","value":"1097-0045"}],"subject":[],"published":{"date-parts":[[2017,7,20]]}}}