{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,13]],"date-time":"2025-11-13T07:10:12Z","timestamp":1763017812168,"version":"3.37.3"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T00:00:00Z","timestamp":1509926400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Sarcosine is a widely discussed oncometabolite of prostate cells. Although several reports described connections between sarcosine and various phenotypic changes of prostate cancer (PCa) cells, there is still a lack of insights on the complex phenomena of its effects on gene expression patterns, particularly in non\u2010malignant and non\u2010metastatic cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>To shed more light on this phenomenon, we performed parallel microarray profiling of RNA isolated from non\u2010malignant (PNT1A), malignant (22Rv1), and metastatic (PC\u20103) prostate cell lines treated with sarcosine. Microarray results were experimentally verified using semi\u2010quantitative\u2010RT\u2010PCR, clonogenic assay, through testing of the susceptibility of cells pre\u2010incubated with sarcosine to anticancer agents with different modes of actions (inhibitors of topoisomerase II, DNA cross\u2010linking agent, antimicrotubule agent and inhibitor of histone deacetylases) and by evaluation of activation of executioner caspases 3\/7.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified that irrespective of the cell type, sarcosine stimulates up\u2010regulation of distinct sets of genes involved in cell cycle and mitosis, while down\u2010regulates expression of genes driving apoptosis. Moreover, it was found that in all cell types, sarcosine had pronounced stimulatory effects on clonogenicity. Except of an inhibitor of histone deacetylase valproic acid, efficiency of all agents was significantly (<jats:italic>P<\/jats:italic>\u2009&lt;\u20090.05) decreased in sarcosine pre\u2010incubated cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our comparative study brings evidence that sarcosine affects not only metastatic PCa cells, but also their malignant and non\u2010malignant counterparts and induces very similar changes in cells behavior, but via distinct cell\u2010type specific targets.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.23450","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T08:01:13Z","timestamp":1509955273000},"page":"104-112","source":"Crossref","is-referenced-by-count":8,"title":["Sarcosine influences apoptosis and growth of prostate cells via cell\u2010type specific regulation of distinct sets of genes"],"prefix":"10.1002","volume":"78","author":[{"given":"Miguel A. Merlos","family":"Rodrigo","sequence":"first","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vladislav","family":"Strmiska","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva","family":"Horackova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hana","family":"Buchtelova","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Michalek","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie","family":"Stiborova","sequence":"additional","affiliation":[{"name":"Department of Biochemistry Faculty of Science Charles University Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Eckschlager","sequence":"additional","affiliation":[{"name":"Department of Paediatric Haematology and Oncology 2<sup>nd<\/sup> Faculty of Medicine Charles University, and University Hospital Motol Prague Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vojtech","family":"Adam","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3915-7270","authenticated-orcid":false,"given":"Zbynek","family":"Heger","sequence":"additional","affiliation":[{"name":"Department of Chemistry and Biochemistry Mendel University in Brno Brno Czech Republic"},{"name":"Central European Institute of Technology Brno University of Technology Brno Czech 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