{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,5]],"date-time":"2025-10-05T19:44:39Z","timestamp":1759693479049,"version":"3.37.3"},"reference-count":67,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,10,8]],"date-time":"2018-10-08T00:00:00Z","timestamp":1538956800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000062","name":"National Institute of Diabetes and Digestive and Kidney Diseases","doi-asserted-by":"publisher","award":["R01 DK099328","U54 DK104310"],"award-info":[{"award-number":["R01 DK099328","U54 DK104310"]}],"id":[{"id":"10.13039\/100000062","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Serum folate concentrations in the United States have risen since dietary folic acid fortification was first mandated in 1998. Although maternal folic acid offers protection against neural tube defects in conceptuses, its impact on other organ systems and life stages have not been fully examined. Here, we used a mouse model to investigate the impact of a Folic acid (FA) enriched diet on prostate homeostasis and response to androgen deprivation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male mice were fed a control diet (4\u2009mg FA\/kg feed) or a folic acid supplemented diet (24\u2009mg FA\/kg feed) beginning at conception and continuing through early adulthood, when mice were castrated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We made the surprising observation that dietary FA supplementation confers partial resistance to castration\u2010mediated prostate involution. At 3, 10, and 14 days post\u2010castration, FA enriched diet fed mice had larger prostates as assessed by wet weight, taller prostatic luminal epithelial cells, and more abundant RNAs encoding prostate secretory proteins than castrated control diet fed mice. Diet did not significantly affect prostate weights of intact mice or serum testosterone concentrations of castrated mice. RNA\u2010Seq analysis revealed that the FA enriched diet was associated with a unique prostate gene expression signature, affecting several signaling and metabolic pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Continuous exposure to a FA enriched diet slows prostate involution in response to androgen deprivation. Prostates from FA diet mice have increased secretory gene expression and increased luminal cell heights. The influence of dietary FA supplementation on the prostate response to androgen deprivation raises a future need to consider how dietary folic acid supplementation affects efficacy of androgen\u2010reducing therapies for treating prostate disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.23723","type":"journal-article","created":{"date-parts":[[2018,10,9]],"date-time":"2018-10-09T05:43:52Z","timestamp":1539063832000},"page":"183-194","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["A folic acid\u2010enriched diet attenuates prostate involution in response to androgen deprivation"],"prefix":"10.1002","volume":"79","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0587-9558","authenticated-orcid":false,"given":"Diya B.","family":"Joseph","sequence":"first","affiliation":[{"name":"Department of Comparative Biosciences University of Wisconsin\u2010Madison Madison 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