{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,29]],"date-time":"2026-08-29T02:48:31Z","timestamp":1787971711691,"version":"build-2784847793"},"reference-count":45,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T00:00:00Z","timestamp":1586217600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81470986"],"award-info":[{"award-number":["81470986"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630019"],"award-info":[{"award-number":["81630019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870519"],"award-info":[{"award-number":["81870519"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2020,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Depressive symptoms are found in approximately 78% of chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS) patients, but the pathological mechanisms remain unknown. Increasing evidence suggests that abnormal gut microbiota may play an important role in depression. Thus, we aimed to investigate whether gut microbiota contributes to CP\/CPPS\u2010associated depression by using a mouse model of experimental autoimmune prostatitis (EAP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male nonobese diabetic mice were immunized twice by subcutaneous injection of prostate antigen and adjuvant. Behavioral tests consisted of an open field test, sucrose preference test, forced swimming tests, and tail suspension test was used to confirm the depression\u2010like symptoms that were induced by EAP. Then, fecal samples were collected, and 16S ribosomal RNA gene sequencing was performed to detect differences in gut microbiota composition between control and EAP group. Additionally, fecal bacteria from the control and EAP mice were transplanted into antibiotics\u2010induced pseudo\u2010germ\u2010free mice to investigate the effects on host behaviors and the composition of gut bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>EAP was successfully established and exhibited depressive\u2010like behaviors in mice. The 16S rRNA analysis of fecal samples indicated the abnormal composition of gut microbiota in the EAP mice compared to the control mice. In the fecal microbiota transplant study, antibiotics\u2010treated pseudo\u2010germ\u2010free mice presented depressive states as compared to na\u00efve mice. Fecal bacteria transplant from EAP mice, but not from control mice, into the pseudo\u2010germ\u2010free mice, significantly exaggerated host depression\u2010like behaviors. Moreover, fecal bacteria transplants from control and EAP mice induced distinct alterations in \u03b1\u2010diversity and \u03b2\u2010diversity indices. In all, 24 bacteria at six phylogenetic levels were remarkably changed by the fecal bacteria transplantation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Abnormal gut microbiota composition after EAP induction may contribute to the development of depression in mice. A therapeutic strategy that targets gut microbiota may provide an alternative treatment for alleviating this condition.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.23978","type":"journal-article","created":{"date-parts":[[2020,4,7]],"date-time":"2020-04-07T13:35:39Z","timestamp":1586266539000},"page":"663-673","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":27,"title":["Abnormal gut microbiota composition is associated with experimental autoimmune prostatitis\u2010induced depressive\u2010like behaviors in mice"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3931-2469","authenticated-orcid":false,"given":"He\u2010Xi","family":"Du","sequence":"first","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-6084","authenticated-orcid":false,"given":"Li\u2010Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chang\u2010Sheng","family":"Zhan","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6695-3013","authenticated-orcid":false,"given":"Jing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4935-4005","authenticated-orcid":false,"given":"Meng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xian\u2010Guo","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chao\u2010Zhao","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Urology, the First Affiliated Hospital of Anhui Medical University Anhui Medical University  Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University  Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University  Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2020,4,7]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.08.046"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2012.08.088"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2009.04.011"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000201"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpsychores.2014.09.012"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/00002508-199409000-00008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2007.10.084"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpain.2006.03.006"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep28608"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/da.22936"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2019.04.006"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2019.00098"},{"key":"e_1_2_8_14_1","first-page":"1","article-title":"A review of dietary and microbial connections to depression, anxiety, and stress","volume":"9","author":"Taylor AM","year":"2018","journal-title":"Nutr Neurosci"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1176\/appi.neuropsych.15120410"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2018.04.038"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1017\/S1041610219000115"},{"key":"e_1_2_8_18_1","doi-asserted-by":"crossref","first-page":"358","DOI":"10.1038\/s41591-019-0396-4","article-title":"Depression linked to the microbiome","volume":"25","author":"Stower H","year":"2019","journal-title":"Nat Med"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2016.02.2959"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1097\/j.pain.0000000000000405"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.1998.0248"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpregu.00836.2007"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2013.11.025"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00187257"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/0014-2999(79)90366-2"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00428203"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.23252"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.18632\/aging.101978"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2170"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.f.303"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btr381"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.00062-07"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrendo.2017.51"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2017.04.022"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbr.2010.03.009"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-019-0379-8"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41398-019-0571-x"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.2147\/NDT.S211288"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20180794"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-40140-5"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecco-jcc\/jjw096"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jad.2019.09.064"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cgh.2019.09.022"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12866-018-1373-7"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1093\/bib\/bbz034"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.23978","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.23978","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pros.23978","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.23978","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T05:08:59Z","timestamp":1694063339000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.23978"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,7]]},"references-count":45,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2020,6]]}},"alternative-id":["10.1002\/pros.23978"],"URL":"https:\/\/doi.org\/10.1002\/pros.23978","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,4,7]]},"assertion":[{"value":"2019-12-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-03-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-04-07","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}