{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T02:23:06Z","timestamp":1740104586330,"version":"3.37.3"},"reference-count":29,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T00:00:00Z","timestamp":1600300800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["U54 DK112079"],"award-info":[{"award-number":["U54 DK112079"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["KAKENHI15K20097"],"award-info":[{"award-number":["KAKENHI15K20097"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2020,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Dutasteride administration reportedly improves lower urinary tract symptoms in patient with chronic, histologically\u2010identified prostatic inflammation, potentially through estrogen receptor \u03b2 (ER\u03b2), activation of which has anti\u2010inflammatory effects in the prostate tissue. Therefore, we investigated the effect of dutasteride on intraprostatic inflammatory responses and bladder activity using a rat model of chemically induced prostatic inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Male Sprague\u2013Dawley rats at 10 weeks old were used. Prostatic inflammation was induced by 5% formalin injection into ventral lobes of the prostate and saline was injected in the control group (control, <jats:italic>n<\/jats:italic>\u2009=\u20095). Rats with prostatic inflammation were divided into dutasteride therapy (dutasteride, <jats:italic>n<\/jats:italic>\u2009=\u20095) and placebo groups (placebo, <jats:italic>n<\/jats:italic>\u2009=\u20095). Dutasteride was administrated at a dose of 0.5\u2009mg\/kg daily from 2 days before induction of prostatic inflammation whereas placebo rats received vehicle only. Twenty\u2010eight days later, cystometry was performed in a conscious condition to measure non\u2010voiding contractions (NVCs), intercontraction intervals (ICI) and postvoid residual volume (RV). After cystometry, the prostate was excised for analysis of messenger RNA (mRNA) expression levels of ER\u03b1, ER\u03b2, interleukin\u20101\u03b2 (IL\u20101\u03b2), and IL\u201018 by quantitative polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mean number of NVCs was significantly greater in placebo group than that of control group without prostatic inflammation (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), and ICI were significantly decreased in placebo group compared with control group (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05). On the contrary, there was no significant change in NVCs or ICI between control and dutasteride groups. RV was not significantly different among three groups. Gene expression levels of ER\u03b1, IL\u20101\u03b2, and IL\u201018 was significantly increased in placebo rats compared with control rats (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), but not significantly different between control and dutasteride rats. On the other hand, the mRNA expression level of ER\u03b2 was significantly decreased in placebo rats (<jats:italic>p<\/jats:italic>\u2009&lt;\u2009.05), but not in dutasteride rats, compared with control rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Dutasteride treatment improved not only prostatic inflammation evident as increased gene expression levels in IL\u20101\u03b2 and IL\u201018, but also bladder overactivity shown by increased NVCs during bladder filling. These therapeutic effects were associated with the restored expression of anti\u2010inflammatory ER\u03b2. Therefore, dutasteride might be effective via ER\u03b2 modulation for the treatment of prostatic inflammation in addition to its previously known, anti\u2010androgenic effects on benign prostatic hyperplasia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.24071","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T19:04:34Z","timestamp":1600369474000},"page":"1413-1420","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Effects of dutasteride in a rat model of chemically induced prostatic inflammation\u2014Potential role of estrogen receptor \u03b2"],"prefix":"10.1002","volume":"80","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0498-4495","authenticated-orcid":false,"given":"Shinsuke","family":"Mizoguchi","sequence":"first","affiliation":[{"name":"Departments of Urology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Urology Oita University Graduate School of Medicine  Yufu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kenichi","family":"Mori","sequence":"additional","affiliation":[{"name":"Departments of Urology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Department of Urology Oita University Graduate School of Medicine  Yufu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toshitaka","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Urology Oita University Graduate School of Medicine  Yufu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhou","family":"Wang","sequence":"additional","affiliation":[{"name":"Departments of Urology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Departments of Pharmacology &amp; Chemical Biology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Donald B.","family":"DeFranco","sequence":"additional","affiliation":[{"name":"Departments of Pharmacology &amp; Chemical Biology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8070-1664","authenticated-orcid":false,"given":"Naoki","family":"Yoshimura","sequence":"additional","affiliation":[{"name":"Departments of Urology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"},{"name":"Departments of Pharmacology &amp; Chemical Biology University of Pittsburgh School of Medicine  Pittsburgh Pennsylvania USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromitsu","family":"Mimata","sequence":"additional","affiliation":[{"name":"Department of Urology Oita University Graduate School of Medicine  Yufu Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2020,9,17]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2006.12.011"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.06.038"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1464-410X.2007.06910.x"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000127760.87421.e9"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000139320.78673.2a"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.eururo.2007.11.026"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.05.071"},{"issue":"1","key":"e_1_2_8_9_1","doi-asserted-by":"crossref","first-page":"274 e271","DOI":"10.1016\/j.urology.2014.09.024","article-title":"Histologic evaluation of human benign prostatic hyperplasia treated by dutasteride: a study by xenograft model with improved severe combined immunodeficient mice","volume":"85","author":"Tsujimura A","year":"2015","journal-title":"Urology"},{"issue":"1","key":"e_1_2_8_10_1","doi-asserted-by":"crossref","first-page":"126","DOI":"10.1177\/1557988315602961","article-title":"Steroid hormone receptors as potential mediators of the clinical effects of dutasteride: a prospective, randomized, double\u2010blind study","volume":"11","author":"Alonso JC","year":"2017","journal-title":"Am J Mens Health"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.23320"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.ju.0000138055.01611.13"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65931-9"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1113\/JP277452"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.23794"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.22668"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.21034"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12950-015-0082-3"},{"issue":"4","key":"e_1_2_8_19_1","first-page":"e211","article-title":"Inflammasome activation leads to IL\u201018 expression in prostatic inflammation associated with BPH","volume":"197","author":"Tyagi P","year":"2017","journal-title":"J Urol"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.58"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2009.04360.x"},{"issue":"16","key":"e_1_2_8_22_1","first-page":"6089","article-title":"Estrogen imprinting of the developing prostate gland is mediated through stromal estrogen receptor alpha: studies with alphaERKO and betaERKO mice","volume":"61","author":"Prins GS","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.steroids.2007.10.013"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0094209"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.fertnstert.2013.02.052"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2010.01.002"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.juro.2011.03.026"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jsbmb.2014.08.003"},{"key":"e_1_2_8_29_1","doi-asserted-by":"crossref","first-page":"25878","DOI":"10.1038\/srep25878","article-title":"Sex differences in the serum level of endogenous ligands for estrogen receptor beta in the elderly population","volume":"6","author":"Kobayashi M","year":"2016","journal-title":"Sci Rep"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1530\/JME-18-0150"}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.24071","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/pros.24071","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.24071","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,4]],"date-time":"2023-09-04T04:49:08Z","timestamp":1693802948000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.24071"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,9,17]]},"references-count":29,"journal-issue":{"issue":"16","published-print":{"date-parts":[[2020,12]]}},"alternative-id":["10.1002\/pros.24071"],"URL":"https:\/\/doi.org\/10.1002\/pros.24071","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"type":"print","value":"0270-4137"},{"type":"electronic","value":"1097-0045"}],"subject":[],"published":{"date-parts":[[2020,9,17]]},"assertion":[{"value":"2020-05-03","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-08-31","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-09-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}