{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T23:46:10Z","timestamp":1790984770795,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"15","license":[{"start":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T00:00:00Z","timestamp":1629504000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630019"],"award-info":[{"award-number":["81630019"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870519"],"award-info":[{"award-number":["81870519"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2021,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Chronic prostatitis\/chronic pelvic pain syndrome (CP\/CPPS) is a common male genitourinary system disease. As a neuroendocrine hormone, melatonin possesses a variety of biological functions, among which its anti\u2010inflammatory effects have recently drawn substantial attention. The purpose of the current research was to study the effect of melatonin on CP\/CPPS and the underlying mechanisms using a mouse model of experimental autoimmune prostatitis (EAP).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The EAP mouse model was successfully established by subcutaneously injecting a mixture of prostate antigen and complete Freund's adjuvant. On Day 42, hematoxylin\u2010eosin staining was used to evaluate the histological appearance of prostate tissues. Chronic pelvic pain development was assessed by suprapubic allodynia. The levels of inflammation\u2010related cytokines, such as interferon\u2010\u03b3, interleukin (IL)\u201017, and IL\u20101\u03b2, were detected by enzyme\u2010linked immunosorbent assay. Then, we explored the anti\u2010inflammatory effects of melatonin on CP\/CPPS by Western blotting and immunohistochemical staining, by measuring the expression of silent information regulator 1 (Sirt1) and NLRP3 inflammasome\u2010related proteins in EAP mice.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>The EAP model mice exhibited severe diffuse leukocyte infiltration and significantly increased pelvic pain compared to the control mice. In the melatonin treatment group, the histological appearance of the prostate tissues, pelvic pain development, and the levels of proinflammatory cytokines were significantly alleviated compared to the EAP\u2009+\u2009dimethyl sulfoxide group. Furthermore, we found that the protective effects of melatonin were achieved through activation of the Sirt1 pathway and downregulation of the NLRP3 inflammasome.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The results indicated that melatonin could attenuate prostate inflammation and pelvic pain by inhibiting the NLRP3 inflammasomes signaling pathway through the activation of Sirt1 in mice with EAP, and these efforts should provide a promising therapeutic strategy for CP\/CPPS.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.24214","type":"journal-article","created":{"date-parts":[[2021,8,21]],"date-time":"2021-08-21T16:49:44Z","timestamp":1629564584000},"page":"1179-1190","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":31,"title":["Melatonin attenuates prostatic inflammation and pelvic pain via Sirt1\u2010dependent inhibition of the NLRP3 inflammasome in an EAP mouse model"],"prefix":"10.1002","volume":"81","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6695-3013","authenticated-orcid":false,"given":"Jing","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory of Genitourinary Diseases Anhui Medical University Hefei Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7520-6084","authenticated-orcid":false,"given":"Li\u2010Gang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Urology The First Affiliated Hospital of Anhui Medical University Hefei Anhui China"},{"name":"Institute of Urology Anhui Medical University Hefei Anhui China"},{"name":"Anhui Province Key Laboratory 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