{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T19:12:50Z","timestamp":1770491570956,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2021,10,5]],"date-time":"2021-10-05T00:00:00Z","timestamp":1633392000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102018R1D1A1A09083833"],"award-info":[{"award-number":["NRF\u20102018R1D1A1A09083833"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2022,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Because of structural alterations in the corpus cavernosum after radical prostatectomy (RP), post\u2010RP erectile dysfunction remains a very difficult condition to treat. We aimed to determine if the combined administration of a Jun\u2010amino terminal kinase (JNK) inhibitor and hepatocyte growth factor (HGF) in the immediate post\u2010injury period would restore erectile function by antiapoptotic and pro\u2010regenerative effects through the rectification of molecular pathways related to the structural integrity of the penis in a rat model of bilateral cavernosal nerve crush injury (CNCI).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 70 rats were divided into five groups: Sham surgery (S), CNCI (I), and once\u2010daily intraperitoneal administration of 10.0\u2009mg\/kg JNK inhibitor\u2009+\u2009twice\u2010weekly intracavernosal administration of low\u2010dose (2.1\u2009\u03bcg), medium\u2010dose (4.2\u2009\u03bcg), or high\u2010dose (8.4\u2009\u03bcg) HGF (I\u2009+\u2009J\u2009+\u2009LH or I\u2009+\u2009J\u2009+\u2009MH or I\u2009+\u2009J\u2009+\u2009HH, respectively) in the immediate post\u2010injury period. Erectile responses to electrostimulation (1.0, 3.0, and 5.0\u2009V), histological staining, caspase\u20103 activity, and Western blotting were evaluated 9 days after surgery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Group I showed lower intracavernosal pressure (ICP)\/mean arterial pressure (MAP) after stimulation at each voltage, lower area under the curve (AUC)\/MAP after stimulation at each voltage, less smooth muscle (SM) content, a lower SM\/collagen ratio, higher caspase\u20103 activity, increased cJun phosphorylation, decreased protein expression of PECAM\u20101, decreased cMet phosphorylation, and decreased endothelial nitric oxide synthase\u00a0(eNOS) phosphorylation compared to Group S. The SM content, SM\/collagen ratio, protein expression of ICP\/MAP, or AUC\/MAP after stimulation at each voltage in Group I\u2009+\u2009J\u2009+\u2009LH were partially restored, despite the normalization of cJun phosphorylation and caspase\u20103 activity. The ICP\/MAP, AUC\/MAP, caspase\u20103 activity, SM content, protein expression of PECAM\u20101, cJun phosphorylation, cMet phosphorylation, and eNOS phosphorylation in both Groups I\u2009+\u2009J\u2009+\u2009MH and I\u2009+\u2009J\u2009+\u2009HH were restored to the levels observed in Group S, while the SM\/collagen ratio was significantly improved but not completely normalized.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our data indicated that the combined administration of a JNK inhibitor and medium or high\u2010dose HGF to nerve\u2010injured rats in the immediate post\u2010injury period after CNCI may restore erectile function to a level comparable to the normal level by suppressing cavernosal apoptosis and preserving the integrity of SM or endothelium via rectification of the cJun and cMet\/eNOS pathways.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.24247","type":"journal-article","created":{"date-parts":[[2021,10,7]],"date-time":"2021-10-07T23:29:28Z","timestamp":1633649368000},"page":"49-58","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Restoration of erectile function by a combination of antiapoptosis by JNK inhibitor and preservation of smooth muscle or endothelium by hepatocyte growth factor in a rat model of cavernous nerve injury"],"prefix":"10.1002","volume":"82","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5519-2490","authenticated-orcid":false,"given":"Soo Woong","family":"Kim","sequence":"first","affiliation":[{"name":"Department of Urology, \u00a0Seoul National University Hospital Seoul\u00a0National University College of Medicine Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Junghoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Urology Seoul National University Boramae Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sohee","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Biostatistics Seoul National University Boramae Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5033-0153","authenticated-orcid":false,"given":"Hwancheol","family":"Son","sequence":"additional","affiliation":[{"name":"Department of Urology Seoul National University Boramae Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7288-1706","authenticated-orcid":false,"given":"Min Chul","family":"Cho","sequence":"additional","affiliation":[{"name":"Department of Urology Seoul National University Boramae Medical Center Seoul South Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2021,10,5]]},"reference":[{"key":"e_1_2_10_2_1","doi-asserted-by":"publisher","DOI":"10.5152\/tud.2019.18488"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.5534\/wjmh.2016.34.2.73"},{"key":"e_1_2_10_4_1","doi-asserted-by":"publisher","DOI":"10.1097\/MOU.0b013e3283136462"},{"key":"e_1_2_10_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/01.mou.0000250286.60237.a6"},{"key":"e_1_2_10_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1743-6109.2008.01105.x"},{"key":"e_1_2_10_7_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra1300575"},{"key":"e_1_2_10_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/bju.12228"},{"key":"e_1_2_10_9_1","first-page":"CD012414","article-title":"Penile rehabilitation for postprostatectomy erectile dysfunction","volume":"10","author":"Philippou 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