{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T22:42:50Z","timestamp":1790808170106,"version":"4.1.0"},"reference-count":31,"publisher":"Wiley","issue":"16","license":[{"start":{"date-parts":[[2024,9,15]],"date-time":"2024-09-15T00:00:00Z","timestamp":1726358400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      The diagnosis of treatment\u2010related neuroendocrine prostate cancer (t\u2010NEPC) often involves a pathological assessment and immunohistochemistry (IHC) for neuroendocrine markers. Genomic alterations in\n                      <jats:italic>RB1<\/jats:italic>\n                      and\n                      <jats:italic>TP53<\/jats:italic>\n                      are frequently observed in NEPC and are believed to play a crucial role in the transformation of adenocarcinoma to NEPC. In this study, we examined the clinicopathologic, immunohistochemical, and genetic features of patients with t\u2010NEPC to better understand their prognosis and diagnostic utility.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      This retrospective study reviewed the records of patients diagnosed with t\u2010NEPC at Kobe University Hospital between October 2018 and December 2022. Clinical data, including age, serum neuroendocrine marker levels, and treatment history, were collected. IHC was performed for conventional neuroendocrine markers (synaptophysin, chromogranin A, and CD56) and RB1 and p53 expression. Next\u2010generation sequencing (NGS) was conducted using FoundationOne\u00ae CDx to identify mutations in RB1 and\n                      <jats:italic>TP53<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      This study included 20 patients with t\u2010NEPC. The median time from ADT initiation to development was 42.8 months. IHC revealed RB1 loss in 75% of cases and p53 abnormalities in 75% of cases. NGS identified\n                      <jats:italic>RB1<\/jats:italic>\n                      mutations in 55% and\n                      <jats:italic>TP53<\/jats:italic>\n                      mutations in 75% of cases. The concordance between NGS and IHC results was high, with 70% (14\/20) agreement for RB1\/\n                      <jats:italic>RB1<\/jats:italic>\n                      and 80% (16\/20) for p53\/\n                      <jats:italic>TP53<\/jats:italic>\n                      . The immunostaining and genomic analysis of RB1\/\n                      <jats:italic>RB1<\/jats:italic>\n                      and p53\/\n                      <jats:italic>TP53<\/jats:italic>\n                      showed abnormal findings for the four negative cases for conventional neuroendocrine markers.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      This study indicated high concordance between IHC and NGS findings for RB1\/\n                      <jats:italic>RB1<\/jats:italic>\n                      and p53\/\n                      <jats:italic>TP53<\/jats:italic>\n                      in t\u2010NEPC. We provide a comprehensive benchmark of NGS performance compared with IHC, and these findings may help increase the diagnostic sensitivity of t\u2010NEPC.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/pros.24791","type":"journal-article","created":{"date-parts":[[2024,9,16]],"date-time":"2024-09-16T02:04:29Z","timestamp":1726452269000},"page":"1506-1514","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Evaluating RB1 and p53 as diagnostic markers in treatment\u2010related neuroendocrine prostate cancer through immunohistochemistry and genomic analysis of\n                    <i>RB1<\/i>\n                    and\n                    <i>TP53<\/i>"],"prefix":"10.1002","volume":"84","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7742-4528","authenticated-orcid":false,"given":"Hideto","family":"Ueki","sequence":"first","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoe","family":"Jimbo","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4447-6516","authenticated-orcid":false,"given":"Tomoaki","family":"Terakawa","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4524-2815","authenticated-orcid":false,"given":"Takuto","family":"Hara","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taisuke","family":"Tobe","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junichiro","family":"Hirata","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoto","family":"Wakita","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuyoshi","family":"Okamura","sequence":"additional","affiliation":[{"name":"Department of Urology Kobe University Graduate School of Medicine Kobe 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