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Non\u2010POU domain\u2010containing octamer\u2010binding (NONO) is involved in RNA transport and almost all steps of gene regulation, and aberrant expression is associated with tumorigenesis in various tumor entities.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Immunohistochemical analysis of the expression of NONO was performed in a cohort of 405 patient samples, including 54 benign prostate tissues, 250 primary prostate tumors, and 101 metastatic tissue samples. To assess the role of NONO in PCa cells, siRNA\u2010mediated knockdown of NONO in the metastatic PCa cell lines PC3 and DU145, followed by a series of functional assays including proliferation, transwell, western blotting, and real\u2010time quantitative PCR, was used.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The current study showed that the nuclear expression of NONO increases during the progression of PCa and is highest in distant metastases. NONO regulates cell proliferation by increasing the expression of cell cycle\u2010related genes. In addition, NONO modulates migration and invasion in PCa cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Overall, these results suggest inhibition of NONO may be a promising approach for the treatment of metastatic PCa.<\/jats:p><\/jats:sec>","DOI":"10.1002\/pros.24925","type":"journal-article","created":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T01:10:22Z","timestamp":1749777022000},"page":"1121-1133","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Downregulation of NONO Suppresses Proliferation, Migration, and Invasion in Metastatic Prostate Cancer"],"prefix":"10.1002","volume":"85","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-0544-8874","authenticated-orcid":false,"given":"Anna\u2010Lena","family":"Lemster","sequence":"first","affiliation":[{"name":"Institute of Pathology University Hospital Schleswig\u2010Holstein L\u00fcbeck 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