{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T10:42:59Z","timestamp":1697884979126},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":8236,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[1984,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The incidence of prostatic cancer is highly correlated with advanced age, and it has been suggested that changes in androgen binding may be important in age\u2010associated alterations in growth regulatory mechanisms of prostatic epithelial cells. In this study the effects of age on androgen binding characteristics in the dorsolateral prostate glands of young and aged Copenhagen rats were determined and the binding properties in the Dunning R3327\/130 subline of rat prostatic adenocarcinoma were characterized. Tritium\u2010labeled and nonlabeled methyltrienolone analogs (R1881) were used to study the binding properties of 5\u03b1\u2010dihydrotestosterone receptor in the cytosol of tumors and prostate glands. Binding of R1881 was low but specific for the androgen receptor as shown by competition studies in which nonlabeled R1881, 5\u03b1\u2010dihydrostestosterone, and testosterone competed successfully with <jats:sup>3<\/jats:sup>H\u2010R1881 for binding sites, but 17\u03b2\u2010estradiol and low levels of progesterone did not. In Copenhagen dorsolateral prostate, Scatchard analysis suggested a single class of binding sites. In young animals (three to five months) the average binding capacity was 10.36 fmol\/mg cytosol protein with a dissociation constant (K<jats:sub>d<\/jats:sub>) of 2.28 nmol\/L. The dorsolateral prostate of aged rats (11\u201316 months) showed no significant difference in specific binding characteristics as compared to the younger age group. Specific binding of <jats:sup>3<\/jats:sup>H\u2010R1881 in R3327\/130 tumor was saturable with a single class of high\u2010affinity binding sites having an average binding capacity of 64.77 fmol\/mg cytosol protein and a K<jats:sub>d<\/jats:sub> of 2.76 nmol\/L. These data show that the tumor had approximately 6.5 times the number of binding sites as did the normal Copenhagen rat dorsolateral prostate gland. However, no age\u2010related changes were detected through 11\u201316 months of age in the androgen binding characteristics of normal rat dorsolateral prostate gland that could be correlated with the higher concentration of androgen binding sites in the R3327\/130 tumor subline.<\/jats:p>","DOI":"10.1002\/pros.2990050110","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T00:08:48Z","timestamp":1172016528000},"page":"101-111","source":"Crossref","is-referenced-by-count":2,"title":["Androgen receptor binding characteristics in the cytosol of the rat dorsolateral prostate gland and the dunning R\u20103327 prostatic adenocarcinoma"],"prefix":"10.1002","volume":"5","author":[{"given":"David R.","family":"Rowley","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue Ann","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David M.","family":"Lubaroff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Paul M.","family":"Heidger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","first-page":"184","volume-title":"Male Accessory Sex Organs, Structure and Function in Mammals","author":"Brandes D","year":"1974"},{"key":"e_1_2_1_3_2","first-page":"293","article-title":"Studies on prostatic cancer. 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