{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:41:01Z","timestamp":1789879261734,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,20]],"date-time":"2006-07-20T00:00:00Z","timestamp":1153353600000},"content-version":"vor","delay-in-days":7505,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["The Prostate"],"published-print":{"date-parts":[[1986,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Hormone sensitivity, as indicated by the presence of steroid hormone receptors and by the effect of androgens and anti\u2010androgens on the release of proteins by cultured cells of the human prostate tumor cell line lymph node carcinoma of the prostate\u2010fast growing colony (LNCaP\u2010FGC), has been studied.<\/jats:p><jats:p>The growth of the LNCaP\u2010FGC cells were stimulated by androgens in a dose\u2010dependent way. Under optimal conditions the synthetic nonmetabolizable androgen 17\u03b2\u2010hydroxy\u201017\u03b1\u2010methyl\u2010(<jats:sup>3<\/jats:sup>H)\u2010estra\u20104,9,11\u2010trien\u20103\u2010one (R1881) (0.1 nM) stimulated cell growth by approximately 2.3 times. Increasing doses of R1881 (1\u2013100 nM) partly decreased the stimulation of the cell growth. The anti\u2010androgen cyproterone acetate exerted inhibitory effects on cell growth. The nuclear extract of the LNCaP\u2010FGC cells contained 17,000 \u00b1 2,500 (mean \u00b1 SD) KCl\u2010extractable, nuclear androgen receptor sites\/cell. Estrogen and progesterone receptors were not detectable in the nuclear extracts nor in cytosol, indicating that these receptors were absent.<\/jats:p><jats:p>The release of proteins in the culture medium was studied using incorporation of the <jats:sup>35<\/jats:sup>S\u2010methionine, sodium dodecyl sulfate (SDS)\u2010gel electrophoresis, and fluorography. Cells grown in media containing charcoal\u2010stripped fetal calf serum released significantly lower amounts of a protein with an apparent molecular mass of 42,000 daltons. The release of this 42\u2010kD protein could be restored in cells cultured in the presence of 5\u03b1\u2010dihydrotestosterone (0.1\u20131 \u03bcM) or R1881 (0.1\u2013100 nM), whereas the addition of estrogens or corticosteroids had no effect. In the presence of anti\u2010androgens, such as cyproterone acetate and 5,5\u2010dimethyl\u20103\u2010(4\u2010nitro\u20103\u2010(trifluoro\u2010methyl)\u2010phenyl)\u20102,4\u2010imidasolidinedione, inhibitory effects on the release of the 42\u2010kD protein were observed. The observed parallel between the effects of (anti)\u2010androgens on the growth of the LNCaP prostate cells and the release of the 42\u2010kD protein suggests that this protein is involved in the regulation of malignant prostate cell growth.<\/jats:p>","DOI":"10.1002\/pros.2990090305","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T00:50:20Z","timestamp":1172019020000},"page":"247-259","source":"Crossref","is-referenced-by-count":98,"title":["Androgen\u2010dependent growth regulation of and release of specific protein(s) by the androgen receptor containing human prostate tumor cell line LNCaP"],"prefix":"10.1002","volume":"9","author":[{"given":"Els M. J. J.","family":"Berns","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Willem","family":"De Boer","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eppo","family":"Mulder","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-103-5-1742"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(80)90621-2"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(80)80001-5"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.75.8.3786"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(81)90281-8"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1210\/jcem-59-3-564"},{"key":"e_1_2_1_8_2","first-page":"1204","article-title":"Distribution of an estrogen\u2010induced protein with a molecular weight of 24,000 in normal and malignant human tissues and cells","volume":"43","author":"Ciocca DR","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6387908"},{"key":"e_1_2_1_10_2","first-page":"147","volume-title":"Progress in Cancer Research and Therapy","author":"Vignon F","year":"1984"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","unstructured":"VignonF ChambonM RochefortH:Autocrine growth stimulation of MCF\u20107 human breast cancer cells by the purified estrogen regulated 52 KJ glycoprotein. 67th Annual Meeting of the Endocrine Society Baltimore MD Abstract 755 1985.","DOI":"10.1210\/endo-118-4-1537"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)49633-9"},{"key":"e_1_2_1_13_2","first-page":"115","volume-title":"Models for Prostatic Cancer, II","author":"Horoszewicz JS","year":"1980"},{"key":"e_1_2_1_14_2","first-page":"16","article-title":"Establishment and characterization of a human prostatic carcinoma cell line (PC\u20103)","volume":"17","author":"Kaighn ME","year":"1979","journal-title":"Invest Urol"},{"key":"e_1_2_1_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(17)52267-3"},{"key":"e_1_2_1_16_2","first-page":"1809","article-title":"LNCaP model of human prostatic carcinoma","volume":"43","author":"Horoszewicz JS","year":"1983","journal-title":"Cancer Res"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01806485"},{"key":"e_1_2_1_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990050407"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-104-4-1007"},{"key":"e_1_2_1_20_2","doi-asserted-by":"publisher","DOI":"10.1210\/endo-118-2-851"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-2697(76)90527-3"},{"key":"e_1_2_1_22_2","first-page":"293","article-title":"Comparison of immunocytochemical and steroid binding assays for estrogen receptor in human breast tumors","volume":"45","author":"King WJ","year":"1985","journal-title":"Cancer Res"},{"key":"e_1_2_1_23_2","first-page":"161","volume-title":"Campbell's Urology","author":"Coffey DS","year":"1978"},{"key":"e_1_2_1_24_2","first-page":"9","volume-title":"Progress in Cancer Research and Therapy","author":"Raynaud JP","year":"1977"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990070208"},{"key":"e_1_2_1_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910320315"},{"key":"e_1_2_1_27_2","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1515\/9783110885026.563","volume-title":"Molecular Mechanism of Steroid Hormone Action","author":"Mulder E","year":"1985"},{"key":"e_1_2_1_28_2","first-page":"201","volume-title":"Testicular Development, Structure and Function","author":"Wilson EM","year":"1980"},{"key":"e_1_2_1_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(83)90076-X"},{"key":"e_1_2_1_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-4731(79)90281-4"},{"key":"e_1_2_1_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0303-7207(84)90078-9"},{"key":"e_1_2_1_32_2","doi-asserted-by":"crossref","first-page":"160","DOI":"10.1093\/clinchem\/28.1.160","article-title":"Proteins of human urine. II. Identification by two\u2010dimensional electrophoresis of a new candidate marker for prostatic cancer","volume":"28","author":"Edwards JJ","year":"1982","journal-title":"Clin Chem."}],"container-title":["The Prostate"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fpros.2990090305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/pros.2990090305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T10:26:57Z","timestamp":1697884017000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/pros.2990090305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1986,1]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1986,1]]}},"alternative-id":["10.1002\/pros.2990090305"],"URL":"https:\/\/doi.org\/10.1002\/pros.2990090305","archive":["Portico"],"relation":{},"ISSN":["0270-4137","1097-0045"],"issn-type":[{"value":"0270-4137","type":"print"},{"value":"1097-0045","type":"electronic"}],"subject":[],"published":{"date-parts":[[1986,1]]}}}