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Two groups of mature, male Sprague\u2010Dawley rats were injected with cadmium chloride subcutaneously (2 mg\/kg body weight) to induce the synthesis of MT, and were sacrificed 48 h postinjection. From the first group, prostatic tissue from the three lobes was prepared for light microscopy. The indirect peroxidase antiperoxidase procedure was used for MT localization using rabbit anti\u2010MT as the primary antibody. The three lobes of the rat prostate demonstrated a positive reaction for MT, but among the lobes variations in the intensity and localization of the reaction product occurred. Tissues from the second group of animals were prepared for electron microscopy. Using the indirect immunogold labeling technique with anti\u2010MT, MT localization was confirmed in the rough endoplasmic reticulum, secretory vesicles, secretory products, and subepithelial connective tissue. These localizations suggest that MT binds zinc both intra\u2010 and extracellularly, where it may function in zinc storage and metabolism.<\/jats:p>","DOI":"10.1002\/pros.2990090409","type":"journal-article","created":{"date-parts":[[2007,2,21]],"date-time":"2007-02-21T00:48:23Z","timestamp":1172018903000},"page":"397-410","source":"Crossref","is-referenced-by-count":24,"title":["Immunocytochemical localization of metallothionein in the rat prostate gland"],"prefix":"10.1002","volume":"9","author":[{"given":"Ziad M.","family":"Bataineh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Paul M.","family":"Heidger","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sue Ann","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barry G.","family":"Timms","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,20]]},"reference":[{"key":"e_1_2_1_2_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja01574a064"},{"key":"e_1_2_1_3_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj1490733"},{"key":"e_1_2_1_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/0300-483X(81)90034-2"},{"key":"e_1_2_1_5_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1978.234.3.E282"},{"key":"e_1_2_1_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/286295a0"},{"key":"e_1_2_1_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(84)90147-9"},{"key":"e_1_2_1_8_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajpendo.1978.234.4.E399"},{"key":"e_1_2_1_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00914204"},{"key":"e_1_2_1_10_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-160-40414"},{"key":"e_1_2_1_11_2","doi-asserted-by":"crossref","first-page":"2180","DOI":"10.1016\/S0021-9258(19)69757-7","article-title":"Cadmium induced accumulation of MT mRNA in rat liver","volume":"256","author":"Ohi S","year":"1981","journal-title":"J Biol Chem"},{"key":"e_1_2_1_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)69958-8"},{"key":"e_1_2_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0041-008X(74)90169-0"},{"key":"e_1_2_1_14_2","first-page":"76","article-title":"On cadmium in normal human kidneys together with a report on the isolation of metallothionein from livers of cadmium\u2010exposed rabbits","volume":"45","author":"Piscator M","year":"1964","journal-title":"Nord Hyg Tidskr"},{"key":"e_1_2_1_15_2","first-page":"622","article-title":"Production of a low\u2010molecular weight cadmium\u2010binding protein in lung after inhalation of cadmium chloride aerosol","volume":"39","author":"Post C","year":"1980","journal-title":"Fed Proc"},{"key":"e_1_2_1_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(77)91041-5"},{"key":"e_1_2_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/0009-2797(76)90138-1"},{"key":"e_1_2_1_18_2","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1093\/jn\/103.7.964","article-title":"Cadmium toxicity in growing swine","volume":"103","author":"Cousins RJ","year":"1973","journal-title":"J Nutr"},{"key":"e_1_2_1_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0048-9697(78)90059-1"},{"key":"e_1_2_1_20_2","first-page":"459","volume-title":"The Prostatic Cell: Structure and Function","author":"Thomas JA","year":"1981"},{"key":"e_1_2_1_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/pros.2990030105"},{"key":"e_1_2_1_22_2","first-page":"104","volume-title":"Immunocytochemistry","author":"Sternberger LA","year":"1979"},{"key":"e_1_2_1_23_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.7.2301"},{"key":"e_1_2_1_24_2","doi-asserted-by":"publisher","DOI":"10.1177\/30.10.6752263"},{"key":"e_1_2_1_25_2","doi-asserted-by":"publisher","DOI":"10.1002\/jemt.1060010304"},{"key":"e_1_2_1_26_2","first-page":"88","volume-title":"Immunocytochemistry. 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